{"created":"2021-03-01T06:05:14.802235+00:00","id":1575,"links":{},"metadata":{"_buckets":{"deposit":"0514b718-b3a1-4e12-89b0-11ef7a84b788"},"_deposit":{"id":"1575","owners":[],"pid":{"revision_id":0,"type":"depid","value":"1575"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00001575","sets":["453:454","485:486:487"]},"author_link":["4386","4387","4388","4389","4390"],"control_number":"1575","item_1627363077551":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1998","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"411","bibliographicPageStart":"407","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"Bioscience, Biotechnology, and Biochemistry","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"An overexpression system for squalene-hopene cyclase (SHC) was constructed by using the pET3a vector, which is responsible for high expression with help from the strong T7 promoter when incorporated into E. coli BL21 (DE3). Site-directed mutagenesis experiments prove that two amino acid residues of tryptophan and aspartic acid inside the QW-motif 5 resided as active sites.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"4389","nameIdentifierScheme":"WEKO"}],"names":[{"name":"佐藤, 努","nameLang":"ja"}]},{"nameIdentifiers":[{"nameIdentifier":"4390","nameIdentifierScheme":"WEKO"}],"names":[{"name":"星野, 力","nameLang":"ja"}]}]},"item_5_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan Society for Bioscience, Biotechnology, and Agrochemistry","subitem_publisher_language":"en"}]},"item_5_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.1271/bbb.62.407","subitem_relation_type_select":"DOI"}}]},"item_5_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10824164","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-8451","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato, Tsutomu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4386","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kanai, Yoshinori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4387","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hoshino, Tsutomu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4388","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-29"}],"displaytype":"detail","filename":"07_0019.pdf","filesize":[{"value":"860.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"07_0019.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/1575/files/07_0019.pdf"},"version_id":"52db70de-95ef-44b6-b480-a369ea730cb1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"squalene","subitem_subject_scheme":"Other"},{"subitem_subject":"hopene","subitem_subject_scheme":"Other"},{"subitem_subject":"squalene cyclase","subitem_subject_scheme":"Other"},{"subitem_subject":"Alicyclobacillus acidocaldarius","subitem_subject_scheme":"Other"},{"subitem_subject":"site-directed mutagenesis","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Overexpression of squalene-hopene cyclase by the pET vector in Escherichia coli and first identification of tryptophan and aspartic acid residues inside the QW motif as active sites.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Overexpression of squalene-hopene cyclase by the pET vector in Escherichia coli and first identification of tryptophan and aspartic acid residues inside the QW motif as active sites.","subitem_title_language":"en"}]},"item_type_id":"5","owner":"1","path":["454","487"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-04-23"},"publish_date":"2008-04-23","publish_status":"0","recid":"1575","relation_version_is_last":true,"title":["Overexpression of squalene-hopene cyclase by the pET vector in Escherichia coli and first identification of tryptophan and aspartic acid residues inside the QW motif as active sites."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T03:34:20.633510+00:00"}