@article{oai:niigata-u.repo.nii.ac.jp:00015725,
 author = {小川, 祐輔},
 issue = {11-12},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Dec},
 note = {Recently, ^<123>I-β-methyl-iodophenyl pentadecanoic acid (BMIPP) has been used in myocardial scintigraphy to evaluate fatty acid metabolism and myocardial viability. Some investigators reported absence of BMIPP-accumulation in the hearts of few patients with heart diseases. The mechanism and clinical significance of absence of BMIPP-accumulation in heart are not known. CD36, glycoprotein IV expressed in platelet membrane, is a receptor for collagen, thrombospondin, oxidised LDL and long-chain fatty acids. CD36 may play a role in platelet aggregation as well as in formation and progression of arteriosclerosis, and in myocardial fatty acid metabolism. The expression and genetic abnormality of CD36 were investigated in this study in relation to myocardial accumulation of BMIPP. There are two types of platelet CD36 deficiency; types I and II , associated with the absence and presence of CD36 on monocytes, respectively. In 200 patients with various heart diseases in this study, 24 cases revealed CD36 deficiency including 8 type I and 16 type II. No myocardial BMIPP-accumulations were observed in all 8 type I cases, while significant accumulation was seen in all type II cases. Genetic analysis of CD36 was carried in 3 of 8 cases with type I deficiency. Deletions of 52 base inn exon 4 and 70 base in exon 9 were documented in mRNA of CD36 in all 3 cases by means of sequence analysis after RT-PCR. Genomic DNA of CD36 was also analyzed by using a PCR-direct sequence method, and there were two types of point mutation, namely, ^<970>T→C/Ile-Thr (one of 3 cases) and ^<478>C→T/Pro→Ser (other 2 cases). The point mutation at ^<478>T was previously reported by other researchers. This is the first report to show the alternative splicing in exons 4 and 9 and the point mutation at ^<970> C. CD36 deficiency may contribute the pathogenesis of heart diseases and arteriosclerosis through abnormal fatty acid metabolism.},
 pages = {526--536},
 title = {^<123>I-BMIPPの心臓への集積欠損を呈する心疾患症例におけるCD36遺伝子の解析},
 volume = {113},
 year = {1999}
}