{"created":"2021-03-01T06:05:13.986047+00:00","id":1562,"links":{},"metadata":{"_buckets":{"deposit":"51db150e-575c-44fa-87ae-b776c127524f"},"_deposit":{"id":"1562","owners":[],"pid":{"revision_id":0,"type":"depid","value":"1562"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00001562","sets":["453:454","485:486:487"]},"author_link":["4310","4311","4312","4313","4314"],"control_number":"1562","item_1627363077551":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"1670","bibliographicPageStart":"1660","bibliographicVolumeNumber":"66","bibliographic_titles":[{"bibliographic_title":"Bioscience, Biotechnology, and Biochemistry","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The functions of Tyr420 and Leu607 were analyzed by constructing various site-directed mutants. The mutation at position 420 into Ala and Gly gave bicyclic α- and γ-polypodatetraene in significant amounts, but with a trace amount of tricyclic malabaricatriene. The kinetic data for and the product distribution of the Y420F mutant indicate that the major function of Tyr420 is to stabilize the 6/6-fused bicyclic cation. Mutation experiments on Leu607 demonstrate that the appropriate steric bulk size at position 607 is required to strongly bind with the product-like conformation formed during the polycyclization process. Introduction of the bulkiest Tip residue into 420 or 607 led to the production of a novel monocyclic triterpene having the (5R, 6R)-1,5,6-trimethylcyclohexene ring, named neoachillapentaene,indicating that the enzymatic cyclization proceeded via a constrained boat structure. Folding of the squalene molecule into a boat conformation by squalene cyclase has not been reported before.","subitem_description_type":"Abstract"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"4314","nameIdentifierScheme":"WEKO"}],"names":[{"name":"佐藤, 努","nameLang":"ja"}]}]},"item_5_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan Society for Bioscience, Biotechnology, and Agrochemistry","subitem_publisher_language":"en"}]},"item_5_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.1271/bbb.66.1660","subitem_relation_type_select":"DOI"}}]},"item_5_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10824164","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-8451","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato, Tsutomu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4310","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasahara, Shigehiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4311","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamakami, Toshiyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4312","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hoshino, Tsutomu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4313","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-29"}],"displaytype":"detail","filename":"07_0015.pdf","filesize":[{"value":"355.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"07_0015.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/1562/files/07_0015.pdf"},"version_id":"47efc4c9-cbd4-44a0-a92a-e4e406eba939"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"squalene","subitem_subject_scheme":"Other"},{"subitem_subject":"hopene","subitem_subject_scheme":"Other"},{"subitem_subject":"terpene cyclase","subitem_subject_scheme":"Other"},{"subitem_subject":"un-natural natural product","subitem_subject_scheme":"Other"},{"subitem_subject":"Alicyclobacillus acidocaldarius","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Functional analyses of Tyr420 and Leu607 of Alicyclobacillus acidocaldarius squalene-hopene cyclase. Neoachillapentaene, a novel triterpene with the 1, 5, 6-trimethylcyclohexene moiety produced through a folding of the constrained boat structure.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Functional analyses of Tyr420 and Leu607 of Alicyclobacillus acidocaldarius squalene-hopene cyclase. Neoachillapentaene, a novel triterpene with the 1, 5, 6-trimethylcyclohexene moiety produced through a folding of the constrained boat structure.","subitem_title_language":"en"}]},"item_type_id":"5","owner":"1","path":["454","487"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-04-23"},"publish_date":"2008-04-23","publish_status":"0","recid":"1562","relation_version_is_last":true,"title":["Functional analyses of Tyr420 and Leu607 of Alicyclobacillus acidocaldarius squalene-hopene cyclase. Neoachillapentaene, a novel triterpene with the 1, 5, 6-trimethylcyclohexene moiety produced through a folding of the constrained boat structure."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T03:34:13.415925+00:00"}