@article{oai:niigata-u.repo.nii.ac.jp:00001551, author = {Uematsu, H. and Sato, N. and Hossain, MD. Z. and Ikeda, T. and Hoshino, E.}, issue = {6}, journal = {Archives of Oral Biology, Archives of Oral Biology}, month = {Jun}, note = {Utilization of a total of 20 amino acids by butyrate-producing asaccharolytic anaerobic Gram-positive rods (AAGPRs) of periodontal pockets, i.e. Eubacterium minutum, Filifactor alocis, E. infirmum, E. sulci and E. saphenum, was studied. E. minutum utilized only arginine and lysine, and produced substantial amounts of butyrate and ammonia from arginine as main metabolic products, while acetate, butyrate and ammonia were from lysine. Fi. alocis utilized only arginine and produced butyrate and ammonia. E. infirmum, E. sulci and E. saphenum utilized only lysine and produced acetate, butyrate and ammonia. The growth of these bacterial species was supported and enhanced by arginine and/or lysine enriched to culture media, but not by the other amino acids. Activities of arginine deiminase, ornithine carbamoyltransferase and carbamate kinase were detected in the cell free extract of E. minutum, suggesting that arginine was metabolised to citrulline, then to ornithine and carbamoyl phosphate. Ornithine and carbamoyl phosphate were further converted to butyrate, and carbon dioxide and ammonia, respectively. While, the enzymatic activity of arginine deiminase and ornithine carbamoyltransferase were not detected in Fi. alocis, indicating that Fi. alocis converted arginine directly to ornithine, not via citrulline, and further to butyrate.}, pages = {423--429}, title = {Degradation of arginine and other amino acids by butyrate-producing asaccharolytic anaerobic Gram-positive rods(AAGPR) of periodontal pockets}, volume = {48}, year = {2003} }