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  1. 050 理学部
  2. 10 学術雑誌論文
  3. 10 査読済論文
  1. 0 資料タイプ別
  2. 01 学術雑誌論文

Classification of the Binding Modes in Bovine Serum Albumin Using Terminally Substituted Alkane Analogues

http://hdl.handle.net/10191/29854
http://hdl.handle.net/10191/29854
f0c017f2-6231-440c-bda9-7fdd4ac3ff87
名前 / ファイル ライセンス アクション
22_12_1571-1575.pdf 22_12_1571-1575.pdf (100.1 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-09-10
タイトル
言語 en
タイトル Classification of the Binding Modes in Bovine Serum Albumin Using Terminally Substituted Alkane Analogues
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Takehara, Ko

× Takehara, Ko

WEKO 4088

en Takehara, Ko

Search repository
Morinaga, Yuki

× Morinaga, Yuki

WEKO 4089

en Morinaga, Yuki

Search repository
Nakashima, Shinya

× Nakashima, Shinya

WEKO 4090

en Nakashima, Shinya

Search repository
Matsuoka, Shiro

× Matsuoka, Shiro

WEKO 4091

en Matsuoka, Shiro

Search repository
抄録
内容記述タイプ Abstract
内容記述 With the fluorescence probe of 8-anilino-1-naphthalenesulfonate (ANS), the binding modes of terminally substituted alkane analogues (CnX; X = COOH, OH, CHO, NH3, CONH2) to bovine serum albumin (BSA) were investigated using a competitive binding technique. The Scatchard plot of the fluorometric titration of BSA with ANS showed that the maximum binding number of ANS, nmax, was 3.81, with the binding constant, Kbnd, of 1.42 × 106 mol-1 dm3. The binding modes of CnX to BSA were analyzed based on the fluorometric titration of the ANS and BSA mixture with CnX. CnCOOH completely displaced the ANS bound to BSA, whereas CnOH and CnCHO displaced only about 40% of the ANS bound to BSA. In contrast, CnNH2 and CnCONH2 displaced very little bound ANS. By comparing these results, we classified the binding modes of CnX to BSA into three types. Two of them are detectable with the ANS fluorescence and the remaining one is not detectable with the fluorescence.
言語 en
書誌情報 en : Analytical sciences

巻 22, 号 12, p. 1571-1575, 発行日 2006-12
出版者
言語 ja
出版者 日本分析化学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 0910-6340
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10500785
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.2116/analsci.22.1571
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
著者版フラグ
値 publisher
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