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Classification of the Binding Modes in Bovine Serum Albumin Using Terminally Substituted Alkane Analogues
http://hdl.handle.net/10191/29854
http://hdl.handle.net/10191/29854f0c017f2-6231-440c-bda9-7fdd4ac3ff87
名前 / ファイル | ライセンス | アクション |
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22_12_1571-1575.pdf (100.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-09-10 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Classification of the Binding Modes in Bovine Serum Albumin Using Terminally Substituted Alkane Analogues | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Takehara, Ko
× Takehara, Ko× Morinaga, Yuki× Nakashima, Shinya× Matsuoka, Shiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | With the fluorescence probe of 8-anilino-1-naphthalenesulfonate (ANS), the binding modes of terminally substituted alkane analogues (CnX; X = COOH, OH, CHO, NH3, CONH2) to bovine serum albumin (BSA) were investigated using a competitive binding technique. The Scatchard plot of the fluorometric titration of BSA with ANS showed that the maximum binding number of ANS, nmax, was 3.81, with the binding constant, Kbnd, of 1.42 × 106 mol-1 dm3. The binding modes of CnX to BSA were analyzed based on the fluorometric titration of the ANS and BSA mixture with CnX. CnCOOH completely displaced the ANS bound to BSA, whereas CnOH and CnCHO displaced only about 40% of the ANS bound to BSA. In contrast, CnNH2 and CnCONH2 displaced very little bound ANS. By comparing these results, we classified the binding modes of CnX to BSA into three types. Two of them are detectable with the ANS fluorescence and the remaining one is not detectable with the fluorescence. | |||||
言語 | en | |||||
書誌情報 |
en : Analytical sciences 巻 22, 号 12, p. 1571-1575, 発行日 2006-12 |
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出版者 | ||||||
言語 | ja | |||||
出版者 | 日本分析化学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0910-6340 | |||||
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収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10500785 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.2116/analsci.22.1571 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
著者版フラグ | ||||||
値 | publisher |