@article{oai:niigata-u.repo.nii.ac.jp:00014862,
 author = {須山, 孝雪},
 issue = {6},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Jun},
 note = {Although dermal fibroblasts receive stretch and relaxation exerted by muscle movement, biological effects of the mechanical force in the cells remains unclear. In the present study to assess effects of stretch and relaxation on cell proliferation and myofibroblast differentiation of dermal fibroblasts, cells were loaded in a biaxial strain device, which was controlled by computer. Cells were subjected to cyclic sigmoidal stretch and relaxation at six cycles/min with 10% maximum change. Immunoblot assay showed that α-smooth muscle actin (α-SMA) expression of cells, either enhanced by transforming growth factor-β_1(TGF-β_1) or not, was decreased by cyclic strain. Flow cytometry suggested that all cells, either stimulated with TG F-β_1 or not, decreased the α-SMA expression in response to the strain. Immunofluorescence microscopy showed that there were star-like, spread cells that were highly positive for α-SMA and spindle cells that were negative or slightly positive for α-SMA, and that the ratio of the former cells to the latter ones was decreased in response to the strain.},
 pages = {257--270},
 title = {Stretch-Relaxationによる真皮線維芽細胞の増殖および分化への影響},
 volume = {115},
 year = {2001}
}