@article{oai:niigata-u.repo.nii.ac.jp:00014772, author = {遠藤, 純男}, issue = {8}, journal = {新潟医学会雑誌, 新潟医学会雑誌}, month = {Aug}, note = {We examined 6 brain lymphomas, pathologically diffuse large B-cell lymphoma, for rearrangements of immunoglobulin heavy chain gene. We amplified the rearranged V_H-D-J_H sequences by RT-PCR, sequenced them, and confirmed their patient-or specimen-specificity by in situ hybridization of individual lymphoma tissues with the use of their own CDR 3 sequences as probes. In subsequent comparative nucleotide analysis and mutation analysis, we found frequent use of V_H 4 gene family (4/6 cases), in particular V 4-34 gene (3/6 cases), and, in all the 6 brain lymphomas, frequent somatic mutations (8.8-27.3%, mean 18.2%) with a significantly low frequency of R (replacement) mutations in the framework regions of V_H gene. These findings suggested that brain lymphomas are related with germinal center lymphocytes, and composed of relatively homogenous tumors in respect of immunological nature.}, pages = {385--396}, title = {脳原発リンパ腫における免疫グロブリン遺伝子の解析}, volume = {115}, year = {2001} }