{"created":"2021-03-01T06:18:41.616929+00:00","id":14268,"links":{},"metadata":{"_buckets":{"deposit":"2677b29f-b5dc-452f-a938-43880e1cde6a"},"_deposit":{"id":"14268","owners":[],"pid":{"revision_id":0,"type":"depid","value":"14268"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00014268","sets":["453:456","471:537:538:1061"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"A Novel  Ca＾2+ dependent Protein-protein Interaction in the Nervous System"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"305","bibliographicPageStart":"299","bibliographicVolumeNumber":"116","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In neurons, exocytosis is performed through the SNARE mechanism. Syntaxin 1A is a key component of the SNARE mechanism. Since exocytosis requires both Ca^<2+> and ATP, we searched for Ca^<2+>/ATP-dependent syntaxin-binding proteins from the rat brain and discovered CaMK II At Ca^<2+> concentrations exceeding 10^<-6>M, only autophosphorylated CaMK II bound to syntaxin. CaMK II bound to the linker domain of syntaxin, unlike any other known syntaxin-binding proteins. CaMK II-syntaxin complexes were also detected in synaptosomes by immunoprecipitation, and when reconstituted in vitro, recruited larger amounts of synaptotagmin and SNAP-25 than syntaxin alone. The microinjected CaMK II-binding domain of syntaxin specifically affected exocytosis in chromaffin cells and in neurons. These results indicate that the Ca^<2+>/ATP-dependent binding of CaMK II to syntaxin is an important process in the regulation of exocytosis.","subitem_description_type":"Abstract"}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"五十嵐, 道弘"}],"nameIdentifiers":[{"nameIdentifier":"92622","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-07"}],"displaytype":"detail","filename":"KJ00000141918.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00000141918.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/14268/files/KJ00000141918.pdf"},"version_id":"af3c5c85-283e-4f83-a94e-6e6c683d2804"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"the SNARE mechanism","subitem_subject_scheme":"Other"},{"subitem_subject":"syntaxin","subitem_subject_scheme":"Other"},{"subitem_subject":"calmodulin-dependent protein kinase II (CaMK II )","subitem_subject_scheme":"Other"},{"subitem_subject":"exocytosis","subitem_subject_scheme":"Other"},{"subitem_subject":"vesicular tarafficking","subitem_subject_scheme":"Other"},{"subitem_subject":"presynaptic terminal","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"神経系における新しいCa<^2+>依存性蛋白質間相互作用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"神経系における新しいCa<^2+>依存性蛋白質間相互作用"},{"subitem_title":"神経系における新しいCa<^2+>依存性蛋白質間相互作用","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1061"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-05-10"},"publish_date":"2007-05-10","publish_status":"0","recid":"14268","relation_version_is_last":true,"title":["神経系における新しいCa<^2+>依存性蛋白質間相互作用"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:46:22.920220+00:00"}