@article{oai:niigata-u.repo.nii.ac.jp:00014268, author = {五十嵐, 道弘}, issue = {7}, journal = {新潟医学会雑誌, 新潟医学会雑誌}, month = {Jul}, note = {In neurons, exocytosis is performed through the SNARE mechanism. Syntaxin 1A is a key component of the SNARE mechanism. Since exocytosis requires both Ca^<2+> and ATP, we searched for Ca^<2+>/ATP-dependent syntaxin-binding proteins from the rat brain and discovered CaMK II At Ca^<2+> concentrations exceeding 10^<-6>M, only autophosphorylated CaMK II bound to syntaxin. CaMK II bound to the linker domain of syntaxin, unlike any other known syntaxin-binding proteins. CaMK II-syntaxin complexes were also detected in synaptosomes by immunoprecipitation, and when reconstituted in vitro, recruited larger amounts of synaptotagmin and SNAP-25 than syntaxin alone. The microinjected CaMK II-binding domain of syntaxin specifically affected exocytosis in chromaffin cells and in neurons. These results indicate that the Ca^<2+>/ATP-dependent binding of CaMK II to syntaxin is an important process in the regulation of exocytosis.}, pages = {299--305}, title = {神経系における新しいCa<^2+>依存性蛋白質間相互作用}, volume = {116}, year = {2002} }