{"created":"2021-03-01T06:15:51.976417+00:00","id":11645,"links":{},"metadata":{"_buckets":{"deposit":"98f1a5f1-7564-4a69-8a7a-a81d8dacd2a1"},"_deposit":{"id":"11645","owners":[],"pid":{"revision_id":0,"type":"depid","value":"11645"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00011645","sets":["453:456","471:537:538:1003"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Nonneuronal Syntaxins Bind to Myosin-V in a Ca^<2+>-dependent Manner"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"272","bibliographicPageStart":"265","bibliographicVolumeNumber":"121","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"目的:Syntaxin familyは細胞内輸送を担う最大のファミリー分子で,神経細胞ではsyntaxin-1Aが開口放出を担っている.非神経細胞には形質膜に局在するsyntaxinアイソフォームが存在し,それぞれの細胞で重要な役割を果たしている.神経細胞では,Ca^<2+>依存性に小胞輸送の分子モーターmyosin-Vがsyntaxin-1Aに結合して,小胞のtetheringに役割を果たす.この機構が非神経型アイソフォームについても成り立つかどうかを,生化学的に検討することにした.方法:組換え蛋白質として,syntaxin-2,3,4とmyosin-Vのそれぞれを作成してin vitroで結合実験を行って,との結合と比較した.またin vitro motilityを行って,myosin-Vの運動性に関する機能制御を解析した.結果:Syntaxin-2,-3,-4は結合の最大量は少ないものの,syntaxin-1Aと同様のmyosin-V結合特性を示した.この相互作用は,mysoin-Vの運動性を抑制しない点は,-1Aの場合と同様であったが,syntaxin-2,3については-1Aのときより,motilityが速くとなるという結果が得られた.副腎髄質クロマフィン細胞において,複数のsyntaxinの存在が確認された.結論:非神経型syntaxinアイソフォームは,神経型のsyntaxin-1Aと同様,myosin-Vと結合することが証明された.これらの結合には細かい差異があり,その点がそれぞれの細胞での各syntaxinアイソフォームの機能的特性,すなわち細胞生物学的な固有の役割を表すと考えられる.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"78792","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ooseki, Koushi"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_relation_31":{"attribute_name":"異版である","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://ci.nii.ac.jp/naid/110006483752","subitem_relation_type_select":"URI"}}]},"item_7_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大関, 康志"}],"nameIdentifiers":[{"nameIdentifier":"78791","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-07"}],"displaytype":"detail","filename":"110006483752.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"110006483752.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/11645/files/110006483752.pdf"},"version_id":"b55472f4-5474-4fb2-9022-4aec32d0859e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"syntaxin","subitem_subject_scheme":"Other"},{"subitem_subject":"myosin-V","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞内小胞輸送","subitem_subject_scheme":"Other"},{"subitem_subject":"開口放出","subitem_subject_scheme":"Other"},{"subitem_subject":"Ca^<2+>","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"非神経型シンタキシン(syntaxin)のミオシンV結合能の性状解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"非神経型シンタキシン(syntaxin)のミオシンV結合能の性状解析"},{"subitem_title":"非神経型シンタキシン(syntaxin)のミオシンV結合能の性状解析","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1003"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-07-30"},"publish_date":"2015-07-30","publish_status":"0","recid":"11645","relation_version_is_last":true,"title":["非神経型シンタキシン(syntaxin)のミオシンV結合能の性状解析"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:44:53.409894+00:00"}