@article{oai:niigata-u.repo.nii.ac.jp:00010708, author = {佐藤, 昇}, issue = {6}, journal = {新潟医学会雑誌, 新潟医学会雑誌}, month = {Jun}, note = {Recent methodological advances using viral and non-viral vectors have allowed for efficient gene delivery into the chicken embryo which is a classical model for studying vertebrate development. Rous sarcoma virus (RSV)-derived replication-competent avian retroviral vectors and in ovo electroporation method can offer efficient transgene expression temporally as well as spatially. Recent progress suggest that gene transfer techniques in combination with the tet regulatory system can allow transgene expression at defind time of development in the chicken embryo. Using these techniques, the action of specific apoptosis-related molecules could be investigated in the developing spinal motoneurons at the time of programmed cell death. Thus, these strategies can provide a valuable tool to address specific biological questions in vertebrate morphogenesis.}, pages = {267--270}, title = {発生脊髄運動ニューロンをラベルする}, volume = {123}, year = {2009} }