2024-03-29T12:15:12Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00020870
2022-12-15T03:51:33Z
453:456
471:537:538:1172
Effects of Hemicranial Irradiation on Myelination in the Neonatal Rat
脳の放射線障害に関する研究 : ラット頭部半側照射モデルにおけるミエリン形成障害
脳の放射線障害に関する研究 : ラット頭部半側照射モデルにおけるミエリン形成障害
佐藤, 光弥
128377
radiation
rat
myelination
myelin basic protein
myelin-associated glycoprotein
照射
ラット
髄鞘形成
髄鞘塩基性蛋白
髄鞘関連糖蛋白
Neonatal rats, aged 4 to 15 days, received single doses of X-irradiation to the right hemicranium. Doses of 5, 7.5, 10 and 15 Gy were administered with linear accelerator. The rats were sacrificed at 14, 21, 28 and 35 days of age and examined for histological changes and immunohistochemically. No remarkable neurological abnormality was noted at the time of sacrifice. With conventional stains there was a dose-dependent decrease in the number of glial cells in the corpus callosum of the irradiated hemisphere, while neurons and blood vessels showed no abnormal changes. Myelin was identified using immunohistochemistry for myelin basic protein and myelin-associated glycoprotein. As compared with unirradiated hemisphere, irradiated hemisphere showed delayed myelination. The antiserum to the large isoform of myelin-associated glycoprotein (L-MAG) stained the cytoplasm of oligodendroglia in 2 to 3 weeks after birth. A smaller number of L-MAG-positive cells were observed in the irradiated hemisphere. This immunohistochemical method appears to be a sensitive histopathological indicator of radiation effect on oligodendroglias. The permeability of the blood-brain barrier (BBB) to sodium fluorescein was investigated and breakdown of the BBB was not observed in the irradiated hemisphere. These results suggest that the delayed myelination in this study was due to a direct radiation effect on oligodendroglias.
departmental bulletin paper
新潟医学会
1992-07
application/pdf
新潟医学会雑誌
7
106
612
625
新潟医学会雑誌
AN00182415
00290440
https://niigata-u.repo.nii.ac.jp/record/20870/files/106(07)_612-625.pdf
jpn