2024-03-28T12:48:32Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00017341
2022-12-15T03:48:42Z
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471:537:538:544
Changes of Electrophoretic Patterns of Urinary Proteins after Running Exercise
走運動による尿蛋白の電気泳動パターンの変化
走運動による尿蛋白の電気泳動パターンの変化
杉本, 英夫
109497
sports medicine
physical exercise
urinalysis
urinary protein
electrophoresis
Western blotting
スポーツ医学
運動負荷
尿分析
尿蛋白
電気泳動
ウエスタン・ブロッティング
For the purpose of a better understanding of the changes of urinary proteins by exercise in healthy subjects, urine samples were collected from 30 healthy male students, 1 hour before (control fraciton), immediately (5-15 min fraction), 1 hour (1 h fraction) and 3 hours (3 h fraction) after 10km running at maximum speed. The samples were studied by measuring the total protein concentrations, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. The followings were observed;1)Total protein concentrations of the control group (control fraction) were 8.4±7.9 (mean±S.D.) mg/dl. 2)The concentrations of the 5-15 min fraction, 1 h fraction and 3 h fraction were 33.4±43.8mg/dl, 53.3±48.2mg/dl and 10.5±4.8mg/dl, respectively. 3)Statistical significance was;control fraction-5-15 min fraction (p<0.01), control fraction-1 h fraction (p<0.01), 5-15 min fraction-1 h fraction (p<0.05), 1 h fraction-3 h fraction (p<0.01), but no significance was seen between control fraction and 3 h fraction. 4)Urinary excretion protein amount at each fraction after running, and their total amount were also studied. 5-15 min fraction was 10.0±13.1mg, 1 h fraction was 26.7±24.1mg and 3 h fraction 5.2±2.4mg. The total amount of urinary protein excretion of 3 hours after 10 km running was 41.2±29.3mg. 5)Statistical study was done for the urinary protein excretion;5-15 min fraction-1 h fraction (p<0.05), 1 h fraction-3 h fraction (p<0.01), 5-15 min fraction-3 h fraction (no significance). 6)Peaks of total protein concentration were seen at 5-15 min fraction or 1 h fraction. Five protein bands (P1, P2, P3, P4 and P5) were seen by SDS-PAGE in almost all cases. P5 was the main band in the fractions except for the peak fractions. 7) By Western blotting, P1 was α2-macroglobulin, P2 was IgG, P4 was transferrin, P5 was albumin and P3 was the derivative of IgG. IgA was also detected in the position of P2.
departmental bulletin paper
新潟医学会
1997-06
application/pdf
新潟医学会雑誌
6
111
362
373
新潟医学会雑誌
AN00182415
00290440
https://niigata-u.repo.nii.ac.jp/record/17341/files/111(6)_362-373.pdf
jpn