2024-03-29T01:11:16Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00016952
2022-12-15T03:48:24Z
453:456
471:537:538:1114
Effects of Temperature and pH on Changes in Intracelluar Ca Ion Concentration of Rat Hippocampal Slices during Oxygen-Glucose Deprivation
低酸素・無グルコース負荷中のラット海馬切片細胞内Caイオン濃度上昇に対する温度とpHの影響
低酸素・無グルコース負荷中のラット海馬切片細胞内Caイオン濃度上昇に対する温度とpHの影響
海老根, 美子
107356
pH
temperature
intracellular Ca ion concentration
ischemia
oxygen-glucose deprivation
温度
細胞内 Ca イオン濃度
虚血
低酸素・無グルコース
Low temperature is commonly known to protect neurons against ischemia, and neuroprotective effect of mild acidosis has also been suggested in our recent reports. In this study, the effects of temperature and pH on ischemic cell damage were studied in terms of intracellular Ca^<2+>, as an indicator of ischemic cell damage, using rat hippocampal slice preparations. Intracellular Ca^<2+> concentration was measured by fluorometry using fura-2. Slices were exposed to ischemia by perfusion with glucose-free artificial CSF (ACSF) bubbled with 95% N_2-5% CO_2. Intracelluar Ca^<2+> showed a characteristic rapid increase at 334±83 sec (n=24) following the start of ischemia at normal temperature (37℃). The latency of the characteristic rapid increase in intracellular Ca^<2+> was prolonged to 535±49 sec (n=9) at low temperature (33℃), while it was shortened to 189±39 sec (n=12) at high temperature (42℃). The latency of the rapid intracellular Ca2+ increase was shortened at each temperature in alkalized ACSF (pH 7.8). The characteristic rapid increase in intracellar Ca^<2+> was not observed at each temperature in acidified ACSF (pH 6.8). The results indicate that both temperature and pH affect the time to the rapid increase in intracellular Ca^<2+> in ischemic tissue, and suggest the neuroprotective effect of hypothermia as well as mild acidosis and also hypothermia plus mild acidosis.
departmental bulletin paper
新潟医学会
1997-11
application/pdf
新潟医学会雑誌
11
111
705
712
新潟医学会雑誌
AN00182415
00290440
https://niigata-u.repo.nii.ac.jp/record/16952/files/111(11)_705-712.pdf
jpn