2024-03-28T09:06:11Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00012679
2022-12-15T03:45:27Z
453:456
471:537:538:1030
Acetaminophen Hepatotoxicity and, CYP2E1 and GSTM1 Genetic Polymorphisms in the Japanese Population
アセトアミノフェンによる肝障害と日本人におけるCYP2E1,GSTM1の遺伝子多型との関連について
アセトアミノフェンによる肝障害と日本人におけるCYP2E1,GSTM1の遺伝子多型との関連について
坂爪, 重明
84408
acetaminophen
hepatotoxicity
CYP2E1
GSTM1
polymorphism
Genetic polymorphisms at -1293, -1053, -352, -333, -71, 1132 and 7632 of CYP2E1 and nonnull or null type of GSTM1 were examined in 101 Japanese patients given 0.3-1.35g of acetaminophen(APAP) orally in Niigata University Medical Hospital and 125 healthy Japanese volunteers using PCR-based analysis. In the CYP2E1 and GSTM1 genotype frequencies there was no dif-ference between the patients and the volunteers(p>0.1). The sites of -71 and 1132 were monomorphic in 226 DNA samples. For Pst I(-1293)/Rsa I(-1053) and Dra I(7632) sites of the CYP2E1 gene, haplotype frequencies were also estimated by a method with Arlequin Software. The outcome of the calculation showed that all variant alleles (CYP2E1^*5), recognized by the Pst I, but not by the Rsa I, carried variant allele A of Dra I sites. At the Dra I sites, 65% of all A alleles carried CYP2E1^*5 allele, which could possibly affect its transcription. Seven of the 101 patients were caused drug-induced liver damage by the APAP ingestion. Of the seven patients, one had developed serious liver damage. There was no difference between hepatotoxicity (+) -patients(n=7) and hepatotoxicity (-) -patients(n=94) either in the CYP2E1 genotype frequencies(p>0.06) or in GSTM1(p=0.84). However, the sample number of the hepatotoxicity (+) -patients is too small to evaluate the association between genotypic differences and liver damage by acetaminophen. Of interest was the observation that the genotype CYP2El^*5/^*5was not found in the hepatotoxicity(+) -patients, but was found in three of the hepatotoxicity(-) -patients. In this study, no association was found between CYP2E1 and GSTM1 polymorphisms examined and APAP-induced liver damage.
departmental bulletin paper
新潟医学会
2005-02
application/pdf
新潟医学会雑誌
2
119
95
105
新潟医学会雑誌
AN00182415
00290440
https://niigata-u.repo.nii.ac.jp/record/12679/files/KJ00004300072.pdf
jpn