2024-03-28T08:39:32Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00006484
2022-12-15T03:39:46Z
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471:537:568:624
HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets
HPLC Application for Phosphoinositides Analyses of A431 Cells and Human Platelets
Kimura, Satoshi
53145
Sugita, Osamu
53146
Matsuto, Takayuki
53147
Okada, Masahiko
53148
Igarashi, Yasuyuki
53149
A new method for the complete separation of polyphosphoinositides with a single high performance liquid chromatography (HPLC) run was applied to human platelets and cell line A431. The amounts of phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP), and phosphatidylinositol 4, 5-bisphosphate (PIP_2) were traced with ^<32>P before and after stimulation. With stimulation by thrombin, increased uptakes of ^<32>P to PI, PIP, and PIP_2 were observed in platelets within 3 min. A similar effect was observed in A431 cells with stimulation by epidermal growth factor. Preincubation with platelet aggregation inhibitors such as hirudin and N, N, N-trimethylsphingosine (an artificial sphingosine derivative) did not produce an increase of PI, PIP, or PIP_2 under stimulation with thrombin. Since phosphoinositides play important roles in signal transduction inside cells, our method should be useful for automatic quantitative analysis and large-scale purification of phosphoinositides.
departmental bulletin paper
Niigata University School of Medicine
1995-03
application/pdf
Acta medica et biologica
1
43
59
62
Acta medica et biologica
AA00508361
05677734
https://niigata-u.repo.nii.ac.jp/record/6484/files/43(1)_59-62.pdf
eng