2024-03-29T12:45:07Z
https://niigata-u.repo.nii.ac.jp/oai
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2022-12-15T03:39:44Z
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471:537:568:621
Frequency of p53-positive Cells in Differing Histological Phases of Ulcerative Colitis
Frequency of p53-positive Cells in Differing Histological Phases of Ulcerative Colitis
Matsuda, Keiji
53009
Watanabe, Hidenobu
53010
Jass, Jeremy R.
53011
Ajioka, Yoichi
53012
Kobayashi, Masaaki
53013
Saito, Hideaki
53014
Sasaki, Masataka
53015
Yasuda, Kazuhiro
53016
Kuwabara, Akifumi
53017
ulcerative colitis
p53 expression
proliferative activity
histological phase
Several reports attest to the absence of p53 immunoreactive cells within non-neoplastic colorectal mucosa, including histologically normal mucosa and inflamed mucosa from subjects with active ulcerative colitis (UC). Failure to detect p53 in these circumstances has been attributed to the short half-life of wild-type p53. Following our previous demonstration of p53 expression in non-neoplastic mucosa, we have studied specimens from ten subjects with ulcerative colitis with the aim of quantifying and correlating this aim with disease activity. The frequency of p53-positive tubules was 19.0% (245/1292) in active UC, 3.1% (46/1487) in resolving and 0.5% (4/863) in UC in remission and 0.3 % (59/17982) in controls. The number of p53-positive cells per tubule with p53-positive cells was 5.4±4.1 in active, 2.4±3.6 in resolving, and 1.3±0.5 in UC in remission and 1.1 ±0.3 in controls. Positive cells occurred singly and were limited to the proliferative zone of tubules in UC and controls. p53 labeling index (LI) in the proliferative zone was 9.0±7.7% in active, 1.5±1.4% in resolving, 1.1±0.5% in UC in remission and 1.2±0.3% in controls. Ki-67 LI in corresponding areas was 68.0± 16.4% in active, 63.1±10.2% in resolving, 48.5±14.4% in UC in remission and 60.9± 10.9% in controls. p53 LI/ Ki-67 LI percentage which is 100% or more in carcinomas with p53 protein overexpression was 14.3± 13.6% (max 52%) in active, 2.24±1.89% in resolving, 2.14±0.73% in UC in remission and 2.03±0.51% in controls. We conclude that p53 immunoreactive cells occur in non-neoplastic conditions and contain wild-type protein. Scattered expression of p53 is not a marker of neoplasia. If p53 immunostaining is to provide a useful diagnostic tool, then the limits of normal and abnormal expression need to be carefully defined.
departmental bulletin paper
Niigata University School of Medicine
1995-12
application/pdf
Acta medica et biologica
4
43
197
203
Acta medica et biologica
AA00508361
05677734
https://niigata-u.repo.nii.ac.jp/record/6454/files/43(4)_197-203.pdf
eng