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2022-12-15T04:31:31Z
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Characteristic Cross Resistance to Anti-Cancer Drugs and Different Responses for Various Growth Factors in Cisplatin Resistant-Human Ovarian Cancer Cell Line (TYK-R10)
Characteristic Cross Resistance to Anti-Cancer Drugs and Different Responses for Various Growth Factors in Cisplatin Resistant-Human Ovarian Cancer Cell Line (TYK-R10)
Kamishima, Tomoko
52917
cisplatin
multidrug resistance
growth factors
ovarian cancer cell line
Cis-diamminedichloroplatinum (II) (CDDP) resistant-human ovarian cancer cell line (TYK-R10) was examined for its drug resistance to other anti-cancer drugs, and growth response to various growth factors, compared with the parental cell line (TYK-nu). TYK-R10 was established by a gradual increase in the concentrations of CDDP compared with the parental line. TYK-R10 showed about a ten-fold resistance, with 1×10^<-5> M CDDP of IC_<50>, which represents the concentration of an anti-cancer drug to produce a 50% inhibition of cell growth. TYK-R10 revealed a cross resistance to various anti-cancer drugs including carboplatin (CBDCA), vincristin (VCR) and adriamycin (ADR). However, no significant alternations in topoisomerase II, glutathione-S-transferase levels or glutathione concentrations were detected. In addition, P-glycoprotein (P-gp) was not expressed on the cell surface of TYK-R10 and verapamil had no reverse effect on resistance to CDDP, CBDCA, VCR and ADR. Surface marker analysis using epidermal growth factor receptor antibody disclosed a high positivity in TYK-R10, compared with TYK-nu, and consequently epidermal growth factor activated the growth of TYK-R10. Transforming growth factor α had no effect on the growth of TYK-R10, whereas it promoted the growth of TYK-nu. In contrast, transforming growth factor β promoted the growth of TYK-nu, whereas it inhibited the growth of TYK-R10. These results indicate that the mechanism of multidrug resistance of TYK-R10 is not mediated through P-gp, and that altered responsibility for some growth factor in TYK-R10 is closely associated with the acquisition of CDDP resistance.
departmental bulletin paper
Niigata University School of Medicine
1996-06
application/pdf
Acta medica et biologica
2
44
79
86
Acta medica et biologica
AA00508361
05677734
https://niigata-u.repo.nii.ac.jp/record/6436/files/44(2)_79-86.pdf
eng