2024-03-29T02:26:18Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00006403
2022-12-15T03:39:41Z
453:456
471:537:568:615
Interactions between the Activation of Phospholipase A_2(PLA_2) and C(PLC) Induced by Thrombin in Human Platelets : Evidence for the Separate Activation of PLA_2 and PLC
Interactions between the Activation of Phospholipase A_2(PLA_2) and C(PLC) Induced by Thrombin in Human Platelets : Evidence for the Separate Activation of PLA_2 and PLC
Fuse, Ichiro
52737
Higuchi, Wataru
52738
Aizawa, Yoshifusa
52739
phospholipase A_2
phospholipase C
G-proteins
human platelets
We investigated the relationship between the activation of phospholipase A_2 (PLA_2) and C (PLC) induced by thrombin in human platelets. Lysophosphatidylinositol (LPI) formation, an indicator of PLA_2 activation, was completely inhibited by pretreatment with pertussis toxin, but inositol-1, 4, 5-triphosphate (IP_3) formation, an indicator of PLC activation, was reduced by about 50%. Prostaglandin E_1 inhibited IP_3 formation completely, but only reduced LPI formation by about 50%. However, cholera toxin did not affect either formation. Conclusion: 1) The differential sensitivity of PLA_2 and PLC to pertussis toxin and PGE_1 strongly suggests that the activity of both enzymes is modulated by distinct GTP- binding proteins; 2) pertussis toxin sensitive G-protein is involved in the thrombin-induced platelet activation process, but cholera toxin is not.
departmental bulletin paper
Niigata University School of Medicine
1997-06
application/pdf
Acta medica et biologica
2
45
45
50
Acta medica et biologica
AA00508361
05677734
https://niigata-u.repo.nii.ac.jp/record/6403/files/45(2)_45-50.pdf
eng