2024-03-28T19:32:29Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00006279
2022-12-15T03:39:36Z
453:456
471:537:568:594
Nosocomial Outbreak-Derived Serratia marcescens : Rapid and Quantitative Detection of the Agent in the Blood by a Real Time PCR Assay
Nosocomial Outbreak-Derived Serratia marcescens : Rapid and Quantitative Detection of the Agent in the Blood by a Real Time PCR Assay
IWAYA, Akira
52084
NAKAGAWA, Saori
52085
IWAKURA, Nobuhiro
52086
TANEIKE, Ikue
52087
HATAKEYAMA, Katsuyoshi
52088
YAMAMOTO, Tatsuo
52089
Serratia marcescens
Real time (TaqMan) PCR
Bacteremia
There have recently been three large nosocomial outbreaks of Serratia marcescens with serious septicaemia and high mortality in Japan. In these blood stream infections, several patients died before the blood culture results were obtained, suggesting that rapid and sensitive methods are needed to detect S. marcescens in the blood. In this study, we examined a rapid DNA diagnosis of outbreak-derived S. marcescens in blood samples by a real time (TaqMan) PCR assay. The minimum level of sensitivity achieved was 5×1O^1 CFU/ml in a buffer, and 1×10^2 CFU/ml in blood samples. The real time PCR assay was completed within 3 h from only 200 μl of blood samples. There was a linear correlation between the bacterial cell number (in a range of 10^2 to 10^7 CFU/ml) and the minimum number of cycling to yield positive results in the assay. The data suggest that a real time (TaqMan) PCR assay is useful for the rapid and quantitative detection of S. marcescens in blood samples.
departmental bulletin paper
Niigata University School of Medicine
2002-12
application/pdf
Acta medica et biologica
4
50
185
190
Acta medica et biologica
AA00508361
05677734
https://niigata-u.repo.nii.ac.jp/record/6279/files/KJ00000007344.pdf
eng