2024-03-28T18:11:52Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00002523
2022-12-15T04:15:26Z
453:454
468:469:470
Adventitious shoot regeneration and micropropagation of the Japanese endangered Hylotelephium sieboldii (Sweet ex Hook.) H. Ohba and H. sieboldii var. ettyuense (Tomida) H. Ohba
Nakano, Masaru
37605
Nagai, Miho
37606
Tanaka, Shigefumi
37607
Nakata, Masashi
37608
Godo, Toshinari
37609
Adventitious shoot
ex situ conservation
Hylotelephium
micropropagation
ornamental plants
Due to indiscriminate collection, the natural habitat of Hylotelephium sieboldii and H. sieboldii var. ettyuense have been significantly reduced. For ex situ conservation and efficient vegetative propagation, a micropropagation system based on adventitious shoot regeneration was developed for these two endangered species. Leaves, stems and roots of in vitro-grown plantlets, and flower buds of greenhouse-grown plants were used as explants. For H. sieboldii, adventitious shoots were most efficiently regenerated from flower bud explants on a medium containing 1 mg l−1 each of NAA and BA. Adventitious shoot regeneration from flower bud explants under this condition was also obtained in H. sieboldii var. ettyuense, but with lower efficiencies. Adventitious shoots of both species rooted and developed into plantlets on a medium containing 0.1 mg l−1 IBA. Almost all of these plantlets were successfully transplanted to the greenhouse. At least at early stage of growth, they showed no apparent morphological alterations.
journal article
日本植物細胞分子生物学会
2005-09
application/pdf
Plant biotechnology
3
22
221
224
AA11250821
1342-4580
https://niigata-u.repo.nii.ac.jp/record/2523/files/22_3_221-224.pdf
eng
http://doi.org/10.5511/plantbiotechnology.22.221