2024-03-28T15:59:54Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00002170
2022-12-15T04:09:34Z
432:477:478
453:454
Pulpal Regeneration after Cavity Preparation, with special Reference to Close Spatio-relationships between Odontoblasts and Immunocompetent Cells
Ohshima, Hayato
14
Nakakura-Ohshima, Kuniko
7380
Takeuchi, Kiichi
7381
Hoshino, Masaaki
7382
Takano, Yoshiro
7383
Maeda, Takeyasu
7384
class II MHC molecule
dental pulp
heat shock protein 25
tissue reaction
The regeneration process of the odontoblast cell layer incident to tooth injury, especially its relationship with immunocompetent cells in pulp healing, has not been fully understood. The purpose of the present study was to clarify this relationship between odontoblasts and immunocompetent cells in the process of pulp regeneration following cavity preparation in rat molars by immunocytochemistry for heat shock protein (Hsp) 25 as well as class II major histocompatibility complex (MHC) molecules. In untreated control teeth, intense Hsp 25-immunoreactivity was found in the cell bodies of odontoblasts and their processes within the predentin, whereas class II MHC-positive cells were predominantly located beneath the odontoblast cell layer. Cavity preparation caused the destruction of the odontoblast layer to form an edematous lesion and the shift of class II MHC-positive cells with the injured odontoblasts toward the pulp core at the affected site. Some damaged odontoblasts without apparent cytoplasmic processes, round in profile, retained the immunoreactivity for Hsp25, suggesting the survival of a part of the odontoblasts against artificial external stimuli. Twelve hours after cavity preparation, numerous class II MHC-positive cells appeared along the pulp-dentin border and extended their processes deep into the exposed dentinal tubules. By postoperative 72 hours, newly-differentiated odontoblasts with Hsp 25-immunoreactivity were arranged at the pulp-dentin border, but the class II MHC-positive cells moved from the pulp-dentin border to the subodontoblastic layer. These findings indicat that the time course of changes in the expression of Hsp 25-immunoreactivity reflects the regeneration process of odontoblasts. The functional roles of Hsp 25-positive odontoblasts and immunocompetent cells such as class II MHC-positive cells in the process of pulp regeneration after cavity preparation are discussed in conjunction with our previous experimental data.
journal article
John Wiley & Sons Ltd.
2003-04
application/pdf
Microscopy research and technique
5
60
483
490
Microscopy research and technique
AA1082314X
1059910X
https://niigata-u.repo.nii.ac.jp/record/2170/files/9_0007.pdf
eng
info:doi/10.1002/jemt.10289
Wiley-Liss, INC.