2024-03-28T11:24:27Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00002161
2022-12-15T04:35:44Z
453:454
482:483:484
Characterization and sensitivity to interleukin-2 and interferoin-alpha of leukemic cells from a patient with large granular lymphocytic leukemia associated with chronic active Epstein-Barr virus infection
Toba, Ken
7330
Koike, Tadashi
7331
Hashimoto, Shigeo
7332
Takahashi, Hoyu
7333
Uesugi, Yumiko
7334
Ishikawa, Toru
7335
Aoki, Sadao
7336
Maeo, Syougo
7337
Naito, Makoto
7338
Aizawa, Yoshifusa
7339
Shibata, Akira
7340
granular lymphocytic leukemia
mosquito allergy
Ebstein-Barr virus
hemophagocytic syndrome
7AAD
PY
A patient presented with chronic large granular lymphocytic leukemia after 13 years of mosquito allergy, recurrent fever, hepato-splenomegaly, liver dysfunction and chronic active EBV infection. Surface marker analysis of the leukemic cells showed the presence of CD2, CD8, CD16 and CD56, and absence of CD3, CD57 and HLA-DR. Cell cycle analysis revealed a minimal growth fraction compatible with chronic lymphocytic leukemia, and Southern blots of the cells showed monoclonal integration of EBV. After 5 months of treatment, the patient died from acute transformation of the leukemia and severe liver dysfunction. Cells harvested during chronic phase were analyzed for sensitivity to interleukin-2 (IL-2) and interferon-alpha (IFNα) in vitro by means of surface phenotyping and cell cycle assay. IL-2 induced remarkable growth of the cells, whereas IFNα did not confer a growth advantage. Since IFNα was expected to have no growth induction effect on the leukemia cells, it was administered to the patient to treat the chronic active EBV infection. There is always a risk of conferring a growth advantage upon cancer cells when growth factors or cytokines are administered in vivo as shown in this report.
journal article
Elsevier
1997-10
application/pdf
Leukemia Research
10
21
941
950
Leukemia Research
AA00716755
0145-2126
https://niigata-u.repo.nii.ac.jp/record/2161/files/24_0022.pdf
eng
http://doi.org/10.1016/s0145-2126(97)00057-x
Elsevier Ltd