2024-03-29T07:16:14Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00002159
2022-12-15T04:18:52Z
453:454
482:483:484
Expression of eosinophil peroxidase in the immature basophil cell line KU812-F
Masuko, Masayoshi
7308
Koike, Tadashi
7309
Toba, Ken
7310
Kishi, Kenji
7311
Kuroha, Takashi
7312
Furukawa, Tatsuo
7313
Fuse, Ichiro
7314
Takahashi, Masuhiro
7315
Kuwano, Ryozo
7316
Shibata, Akira
7317
Aizawa, Yoshifusa
7318
Basophils
Eosinophil peroxidase
Immunocytochemistry
KU812-F
mRNA
Although peroxidase activity in basophils can be detected by optical and ultrastructural cytochemistry, its characteristics remain to be determined. We have demonstrated the characteristics of peroxidase activity induced in the immature basophil cell line, KU812-F. Ultrastructually, peroxidase activity was detected in granules as well as in the perinuclear space and endoplasmic reticulum. Immunocytochemisty revealed that KU812-F cells were stained by anti-eosinophil peroxidase antibodies, and eosinophil peroxidase mRNA, not myeloperoxidase, was detected in the cells using Northern hybridization and reverse transcription-polymerase chain reaction. Eosinophil peroxidase can be one of the molecules shared with eosinophils and basophils. The biological function of eosinophil peroxidase detected in basophils remains uncertain.
journal article
Elsevier
1999-02
application/pdf
Leukemia Research
2
23
99
104
Leukemia Research
AA00716755
0145-2126
https://niigata-u.repo.nii.ac.jp/record/2159/files/24_0014.pdf
eng
http://doi.org/10.1016/s0145-2126(98)00140-4
Elsevier Ltd