2024-03-28T09:43:43Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00001569
2022-12-15T03:34:20Z
453:454
485:486:487
Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27.
Honjoh, Ken-ichi
4350
Matsumoto, Hiroko
4351
Shimizu, Hideyuki
4352
Ooyama, Kanae
4353
Tanaka, Kageyuki
4354
Oda, Yuichi
4355
Takata, Ryoji
4356
Joh, Toshio
4357
Suga, Koushirou
4358
Miyamoto, Takahisa
4359
Iio, Masayoshi
4360
Hatano, Shoji
4361
Chlorella vulgaris C-27
cryoprotection
freezing tolerance
LEA protein
The nucleotide sequence of hiC12, isolated as a cDNA clone of hardening-induced Chlorella (hiC) genes, was identified. The clone encodes a late embryogenesis abundant (LEA) protein having six repeats of a 11-mer amino acid motif, although in a slightly imperfect form. To overexpress the hiC61) and hiC12 genes, their coding regions were PCR amplified and subcloned into a pGEX-1λT vector. The HIC6 and HIC12 proteins were expressed as GST fusion proteins in E. coli, then purified. The two HIC proteins were found to be effective in protecting a freeze-labile enzyme, LDH, against freeze-inactivation. On a molar concentration basis, they were about 3.1×106 times more effective in protecting LDH than sucrose and as effective as BSA. Cryoprotection tests with five kinds of chain-shortened polypeptides, synthesized based on the 11-mer amino acid motif of the HIC6 protein showed that the cryoprotective activity decreased with a decrease in the repeating units of the 11-mer motif. In fact, cryoprotective activities of three kinds of single 11-mer amino acids were very low even at high concentrations. All the results suggested that the sufficiently repeated 11-mer motif is required for the cryoprotective activities of Chlorella LEA proteins.
journal article
Japan Society for Bioscience, Biotechnology, and Agrochemistry
2000
application/pdf
Bioscience, Biotechnology, and Biochemistry
8
64
1656
1663
AA10824164
0916-8451
https://niigata-u.repo.nii.ac.jp/record/1569/files/07_0013.pdf
eng
http://doi.org/10.1271/bbb.64.1656