2024-03-28T09:22:51Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:02000972
2023-04-27T02:14:48Z
453:455
471:561:562
Crumbs3 is expressed in oral squamous cell carcinomas and promotes cell migration and proliferation by affecting RhoA activity
Crumbs3は口腔扁平上皮癌において、RhoA活性化を制御し、細胞の活動性および増殖性を亢進させる
Yokoyama, Yusuke
横山, 侑輔
open access
© Yokoyama et al.
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
crumbs
Crb3
HNSCC
OSCC
squamous cell carcinoma
ras homolog family member A
metastasis
Despite the recent progression of treatments, the 5-year survival rate of patients with oral squamous cell carcinoma (OSCC) is still poor. One of the most critical factors affecting prognosis is tumor metastasis. Developing novel molecular targeted therapies by analyzing the molecular pathway of OSCC metastasis is an urgent issue. The present study aimed to characterize the expression and function of crumbs3 (Crb3) in OSCC cell migration. Immunohistochemistry and immunoblotting revealed that Crb3 was expressed in tissues from patients with OSCC and OSCC cell lines. The motility of OSCC cell lines was decreased by knockdown of Crb3 without affecting proliferation. However, Crb3-knockout (KO) clones exhibited decreases in both cell migration and proliferation. The expression of epithelial-mesenchymal transition markers was not altered in Crb3-KO clones compared with parent cells. A xenograft mouse model of lung metastasis revealed that the metastatic potential of Crb3-KO clones was reduced. As seen with Crb3-KO clones, the motility of OSCC cells was decreased by treatment with inhibitors of RhoA activation. Serum-induced activation of RhoA in OSCC cells was evaluated by comparing the amount of GTP-bound RhoA using affinity matrices, revealing that RhoA activation was decreased in Crb3-KO clones. To the best of our knowledge, the present study was the first to demonstrate that Crb3 was expressed in squamous cell carcinoma tissues and promoted cell migration and proliferation, which was associated with RhoA activation in OSCC cells.
Oncology Letters. 2022, 23, 173.
新大院博(医)第1093号
eng
doctoral thesis
http://hdl.handle.net/10191/0002000972
https://niigata-u.repo.nii.ac.jp/records/2000972
https://doi.org/10.3892/ol.2022.13293
甲第5080号
博士(医学)
2022-09-20
13101
新潟大学
Niigata University
https://niigata-u.repo.nii.ac.jp/record/2000972/files/r4nmk1093.pdf
application/pdf
4.89MB
2023-04-27
https://niigata-u.repo.nii.ac.jp/record/2000972/files/r4nmk1093_a.pdf
application/pdf
435KB
2023-04-27