2024-03-28T14:06:37Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00033915
2022-12-15T04:04:10Z
453:455
471:561:562
Loss of autophagy in chondrocytes causes severe growth retardation
軟骨細胞におけるオートファジー欠損は重度の成長障害を引き起こす
Horigome, Yoji
【○!C】 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Atg7
autophagy
cartilage
chondrogenesis
glycogenolysis
Chondrogenesis is accompanied by not only cellular renovation, but also metabolic stress. Therefore, macroautophagy/autophagy is postulated to be involved in this process. Previous reports have shown that suppression of autophagy during chondrogenesis causes mild growth retardation. However, the role of autophagy in chondrocyte differentiation still largely remains unclear. Here, we show the important role of autophagy on chondrogenesis. The transition of mesenchymal cells to chondrocytes was severely impaired by ablation of Atg7, a gene essential for autophagy. Mice lacking Atg7 after the transition exhibited phenotypes severer than mutant mice in which Atg7 was removed before the transition. Atg7-deficient chondrocytes accumulated large numbers of glycogen granules, hardly proliferate and died specifically in the proliferative zone without any ER-stress signal. Our results suggest that the suppression of autophagy in prechondrogenic cells drives compensatory mechanism(s) that mitigate defective chondrogenesis, and that autophagy participates in glycogenolysis to supply glucose in avascular growth plates.
Autophagy. 2020, 16(3), 501-511.
新大院博(医)甲第892号
eng
thesis
http://hdl.handle.net/10191/00051713
https://niigata-u.repo.nii.ac.jp/records/33915
info:doi/10.1080/15548627.2019.1628541
13101甲第4632号
博士(医学)
2019-09-20
新潟大学
https://niigata-u.repo.nii.ac.jp/record/33915/files/r1nmk892.pdf
application/pdf
4.2 MB
2020-07-08
https://niigata-u.repo.nii.ac.jp/record/33915/files/r1nmk892_a.pdf
application/pdf
639.7 kB
2020-07-08