2024-03-28T22:57:41Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00024672
2022-12-15T03:54:48Z
453:456
471:537:538:1244
ラット脳における小脳酸性蛋白spot35蛋白の発現
ラット脳における小脳酸性蛋白spot35蛋白の発現
Expression of a Cerebellar Protein (Spot 35 Protein) in Rat Brains
山国, 徹
cerebellar protein
mRNA
translation
cDNA
hybridization
expression
development
小脳蛋白
メッセンジャー
RNA
翻訳
相補的DNA
ハイプリダイゼィション
発現
発育
Spot 35 protein is a cerebellar soluble protein with Mr. 27K daltons and pI 5.3, which localizes in Purkinje cells. In order to understand the development of cerebellum at molecular level, it is necessary to reveal the mechanism controlling the biosynthesis of spot 35 protein. Here I examined the developmental and regional changes of mRNA levels for spot 35 protein in rat brain, by using in vitro translation system and dot-blot hybridization with spot 35 protein cDNA as a probe. Dot-blot hybridization analysis revealed that the mRNA in rat cerebellum rapidly increased during postnatal 10-20days and attained a-plateau level after 20days. This result was in agreement with the data of analysis or mRNA level for spot 35 protein by means of in vitro translation system containing rat cerebellar mRNA. The data on spot 35 protein mRNA correlate with the result of immunohistochemical examination using anti-spot 35 protein antiserum. Further, the regions except cerebellum in rat brain contained only negligible Spot 35 protein mRNA. These results suggest a transcriptional control of the concentration of spot 35 protein in developing cerebellum and an intensive expression of its gene in cerebellum.
新潟医学会
1986-07
jpn
departmental bulletin paper
http://hdl.handle.net/10191/34888
https://niigata-u.repo.nii.ac.jp/records/24672
AN00182415
00290440
新潟医学会雑誌
新潟医学会雑誌
100
7
389
397
https://niigata-u.repo.nii.ac.jp/record/24672/files/100(7)_389-397.pdf
application/pdf
1.1 MB
2019-08-19