2024-03-29T05:46:12Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00023984
2022-12-15T03:54:09Z
453:456
471:537:538:1231
1) 遺伝子操作の概略(シンポジウム 遺伝子工学と医学, 第422回新潟医学会)
1) 遺伝子操作の概略(シンポジウム 遺伝子工学と医学, 第422回新潟医学会)
The outline of recombinant DNA technology (Gene Technology and Medicine)
三嶋, 行雄
recombinant DNA technology
DNA cloning
遺伝子操作
DNAクローニング
I introduce the outline of recombinant DNA technology. The most important techniques used in recombinant DNA technology are (1) specific cleavage of DNA by restriction endonucleases, DNA synthesis by DNA polymerase or reverse transcriptase, and DNA ligation by DNA ligase, (2) DNA cloning procedures to utilize plasmids or viruses as a vectorr so that a specific DNA fragment can be amplified in bacteria cells, (3) nucleic acid hybridization to identify the specific sequences and (4) DNA sequencing to determine the nucleotide sequence of cloned DNA fragments.
新潟医学会
1987-08
jpn
departmental bulletin paper
http://hdl.handle.net/10191/36643
https://niigata-u.repo.nii.ac.jp/records/23984
AN00182415
00290440
新潟医学会雑誌
新潟医学会雑誌
101
8
494
497
https://niigata-u.repo.nii.ac.jp/record/23984/files/101(8)_494-497.pdf
application/pdf
329.7 kB
2019-08-19