2024-03-29T06:57:18Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00022679
2022-12-15T03:53:01Z
453:456
471:537:538:1203
6) アレルギー性脳脊髄炎(シンポジウム 自己免疫疾患, 第445回新潟医学会)
6) アレルギー性脳脊髄炎(シンポジウム 自己免疫疾患, 第445回新潟医学会)
Experimental Allergic Encephalomyelitis(Autoimmune Diseases)
松本, 陽
Experimental allergic encephalornyelitis
EAE
Immunohistochemistry
Limiting dilution analysis
Bone marrow chimera
アレルギー性脳髄炎
免疫組織化学
限界稀釈法
骨髄キメラ
Experimental allergic encephalomyelitis (EAE) is a T-cell mediated autoimmune disease inducible by immunization with brain specific antigens such as myelin basic protein (MBP) in complete Freund's adjuvant (CFA). Using EAE as an experimental model for human autoimmune disorders, we show here several experimental approaches and the results obtained by these approaches. They include 1) immunohistochemical examination of the lesions of EAE, 2) double immunofluorescent staining of the tissue in order to identify the nature of cells of interest, 3) passive EAE which facilitutes the examination of developing lesions of EAE, and 4) radiation bone marrow chimeras which were prepared for the detection of bone marrow-derived cells in the central nervous system. We also introduced limiting dilution analysis of MBP-reactive T cells and thymus-grafted chimeras.
新潟医学会
1989-12
jpn
departmental bulletin paper
http://hdl.handle.net/10191/41473
https://niigata-u.repo.nii.ac.jp/records/22679
AN00182415
00290440
新潟医学会雑誌
新潟医学会雑誌
103
12
985
987
https://niigata-u.repo.nii.ac.jp/record/22679/files/103(12)_985-987.pdf
application/pdf
626.5 kB
2019-08-19