2024-03-28T15:08:09Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00022613
2022-12-15T03:52:58Z
453:456
471:537:538:1201
ラット母児間のcharge barrierの局在
ラット母児間のcharge barrierの局在
Localization of the Charge-dependent Materno-fetal Barrier in the Rat
山崎, 一郎
charge barrier
cationic protein
placenta
rat
チャージバリヤー
塩基化蛋白
胎盤
ラット
Near-term rats placentas were directly injected with two pairs of proteins differently charged. The placental localization of the proteins 60 min after the injection was determined by immunohistochemical staining and light microscopic autoradiography. Immunohistochemical studies (ie., immunofluorescence and immunoperoxidase studies) showed that there was no preferential localization in the placenta of native proteins injected. In contrast, cationized proteins injected were localized significantly in the Reichert's membrane. By light microscopic autoradiography, a large number of positive grains were found in the Reichert's membrane which is strongly positive for periodic acid-Schiff stain. And positive grains were also associated with trophoblast, parietal endoderm and visceral endoderm cells following the injection of cationized proteins. However, it is consistent with the immunohistochemical findings that positive grains were observed scarcely in the placenta when native proteins were injected. The present study suggest that Reichert's membrane in the rat may play an important role for the charge-dependent materno-fetal barrier since the rodent parietal yolk sac is well known to serve as a route via placenta.
新潟医学会
1990-02
jpn
departmental bulletin paper
http://hdl.handle.net/10191/33672
https://niigata-u.repo.nii.ac.jp/records/22613
AN00182415
00290440
新潟医学会雑誌
新潟医学会雑誌
104
2
143
147
https://niigata-u.repo.nii.ac.jp/record/22613/files/104(2)_143-147.pdf
application/pdf
3.5 MB
2019-08-16