2024-03-29T07:10:28Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00020867
2022-12-15T03:51:33Z
453:456
471:537:538:1172
ケラチン生物学とその皮膚科学への応用
ケラチン生物学とその皮膚科学への応用
Keratin Biology and its Application to Dermatology
伊藤, 雅章
keratin
SDS gel electrophoresis
monoclonal antibodies
immunohistochemistry
cell differentiation
ケラチン
SDSゲル電気泳動
単クローン抗体
免疫組織化学
細胞分化
Recent advances in keratin biology were summarized and the usefulness of keratin staining and keratin electrophoresis in dermatology was introduced. Anti-hair keratin monoclonal antibodies were produced against human hair keratin filament proteins by mouse hybridoma method. Some of the antibodies showed immunohistochemical reactivities limited within hair tissue and others cross-reacted with hair tissue and other epithelia. The innermost cell layer of the outer root sheath in anagen hair follicle was tmmunohistochemically and ultrastructurally found to be a unique layer showing own cell differentiation. When these monoclenal antibodies and anti-soft keratin monoclonal antibodies were applied on immunohistochemical surveys of various kinds of skin epithelial tumors, the tumors were classified into several types based on their keratin expression. The keratin staining was also useful to investigate the keratinization disorder in a congenital bullous disease. As well as keratins, keratin-related substances such as keratohyalin and trichohyalin may be important for further studies in keratin biology.
新潟医学会
1992-07
jpn
departmental bulletin paper
http://hdl.handle.net/10191/39835
https://niigata-u.repo.nii.ac.jp/records/20867
AN00182415
00290440
新潟医学会雑誌
新潟医学会雑誌
106
7
585
593
https://niigata-u.repo.nii.ac.jp/record/20867/files/106(07)_585-593.pdf
application/pdf
2.8 MB
2019-08-16