2024-03-29T01:17:21Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00020736
2022-12-15T04:23:24Z
453:456
471:537:538:1169
脳腫瘍の温熱療法に関する基礎的研究 : 温熱の脳組織ならびに脳血管透過性に及ぼす影響
脳腫瘍の温熱療法に関する基礎的研究 : 温熱の脳組織ならびに脳血管透過性に及ぼす影響
Hyperthermia in Brain Tumor Therapy : The Effect of Hyperthermia on Brain Tissues and Cerebrovascular Permeability
関原, 芳夫
hyperthermia
brain
blood-brain barrier
immunohistochemistry
温熱療法
脳
脳血液関門
免疫組織化学
The effect of hyperthermia on normal brain tissues and cerebrovascular permeability have recently been reported, but its critical temerature is not yet well determined. The present study was aimed at the effects by two different methods, whole body and RF interstitial heating. Whole body heatings of mongrel dogs were performed with extracorporeal systemic circulation method. The temperature of the brain was continously monitored. The permeability was assessed by injection of 10% fluorescein(FL.) and/or 2% Evans blue (EB) 30 min before sacrifice, followed fluorescence photograph or visual inspection of EB extravasation. Then, using paraffin embedded formalin fixed tissues, immunohistochemical demonstration of the animal's own serum albumin and fibrinogen using the ABC method was performed. No regions in the brain heated at 43℃ for 30 or 60 min showed FL. nor EB extravasation except blood-brain barrier lacking regions and a thermosensor penetrated Site. Immunohistochemical study also showed no abnormal extravasation of serum albumin nor fibrinogen. At the same heating conditions, no histological changes were seen. RF interstitial heatings of mongrel dogs were performed as follows. A RF antenna was inserted into the brain to a depth of 10 mm from the brain surface, and were also done 4 themosensors around the antenna. Monitoring a temperature at a reference point 5~7 mm distent from the antenna, the brain was heated at 42~45℃ for 60 min. The permeability was evaluated by tracer method using EB and immunohistochemistry as described in whole body heating. A 60 min heat treatment produced a round lesion that consisted of central coagulation necrosis surrounded by a well demarcated blue zone which was heated over 44℃. The blue zone dissappeared, and 24 hrs after the treatment, changed into a coagulative/liquefactive necrosis. The vascular pemeability and histological changes were also examined at various intervals from 1 to 30 days. On 7 and 9 days after treatment, faint EB extravasation was temporarily observed at the marginal zone of the necrosis. However, in regions heated at 42~44℃, no changes of vascular permeability nor histological damages without edema were observed. These studies suggest that cerebrovascular permeability is increased over 44℃ for 60 min, then, this permeable area is changed into necrotic lesion during 24 hrs afer the treatment. However, the brain can withstand approximately at temperatures of less than 43.5℃ without changes of vascular permeability nor histological damage.
新潟医学会
1992-10
jpn
departmental bulletin paper
http://hdl.handle.net/10191/40057
https://niigata-u.repo.nii.ac.jp/records/20736
AN00182415
00290440
新潟医学会雑誌
新潟医学会雑誌
106
10
997
1007
https://niigata-u.repo.nii.ac.jp/record/20736/files/106(10)_997-1007.pdf
application/pdf
1.9 MB
2019-08-16