2024-03-28T23:06:43Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00019405
2022-12-15T03:50:20Z
453:456
471:537:538:1151
ActivinA/EDF (Erythroid Differentiation Factor) のヒト造血幹細胞分化に及ぼす影響
ActivinA/EDF (Erythroid Differentiation Factor) のヒト造血幹細胞分化に及ぼす影響
Effects of ActivinA/EDF (Erythroid Differentiation Factor) on Differentiation of Human Hematopoietic Stem Cell
後藤, 隆夫
EDF (erythroid differentiation factor)
erythropoiesis
megakaryocytic differentiation
cyclo-oxygenase
cytokine
赤血球分化
巨核芽球分化
サイクロオキシゲナーゼ
造血因子
ActivinA/EDF (Erythroid Differentiation Factor) purified from the culture fluid of human monocytic cells (THP-1 cells) has shown to be potential to a differentiation murine erythroleukemic cells (Friend cells). In this study, we demonstrated that ActivinA/EDF increases in the number of hemoglobin positive cells in human erythroleukemia cell lines without the reduction of cell proliferation. In addition, it enhances erythroid colony formation from human erythroid progenitors of normal and MDS (myelodysplastic syndrome) in vitro. On the other hand, cyclo-oxygenase (CO) mRNA levels increase in liquid culture of human erythroleukemia cell lines after incubation with ActivinA/EDF by RT-PCR (reverse transcription-polymerase chain reaction). These results suggest that ActivinA/EDF possibly acts as an important cytokine of erythropoiesis and megakaryocytic differentiation of human hematopoietic stem cell.
新潟医学会
1994-04
jpn
departmental bulletin paper
http://hdl.handle.net/10191/38180
https://niigata-u.repo.nii.ac.jp/records/19405
AN00182415
00290440
新潟医学会雑誌
新潟医学会雑誌
108
4
301
308
https://niigata-u.repo.nii.ac.jp/record/19405/files/108(4)_301-308.pdf
application/pdf
1.6 MB
2019-08-08