2024-03-28T12:50:50Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00018891
2022-12-15T03:49:57Z
453:456
471:537:538:1144
Spot 35/calbindin D 28k遺伝子の解析
Spot 35/calbindin D 28k遺伝子の解析
Expression of the Spot 35/calbindin D 28k gene
桑野, 良三
cerebellum
calcium binding protein
transcription
vitamin D response element
小脳
カルシウム結合蛋白
転写
ビタミンD応答配列
Spot 35 protein was first discovered from the cerebellum as a specific protein. Immunohistochemistry demonstrated wide distribution of this protein in the Purkinje cells, intestine, sensory organs and kidney. cDNA cloning and nucleotide sequence showed that Spot 35 protein is identical with calbindin D 28k, a vitamin D dependent calcium binding protein. Two vitamin D response elements (pDRE and dDRE) were found in the 5' upstream region of the Spot 35/Calbindin D 28k gene. Promoter activities were determined by using luciferase as a reporter gene in MDBK cells which produced endogenously Spot 35/Calbindin D 28k. Transcription of the chimeric gene containing the pDRE was enhanced by 1, 25 (OH)_2D_3 stimulation in an orientation-dependent fashion.
新潟医学会
1994-11
jpn
departmental bulletin paper
http://hdl.handle.net/10191/40477
https://niigata-u.repo.nii.ac.jp/records/18891
AN00182415
00290440
新潟医学会雑誌
新潟医学会雑誌
108
11
798
803
https://niigata-u.repo.nii.ac.jp/record/18891/files/108(11)_798-803.pdf
application/pdf
780.2 kB
2019-08-08