2022-11-28T09:49:07Zhttps://niigata-u.repo.nii.ac.jp/oaioai:niigata-u.repo.nii.ac.jp:000173412021-03-01T15:18:47Z走運動による尿蛋白の電気泳動パターンの変化走運動による尿蛋白の電気泳動パターンの変化Changes of Electrophoretic Patterns of Urinary Proteins after Running Exercise杉本, 英夫sports medicinephysical exerciseurinalysisurinary proteinelectrophoresisWestern blottingスポーツ医学運動負荷尿分析尿蛋白電気泳動ウエスタン・ブロッティングFor the purpose of a better understanding of the changes of urinary proteins by exercise in healthy subjects, urine samples were collected from 30 healthy male students, 1 hour before (control fraciton), immediately (5－15 min fraction), 1 hour (1 h fraction) and 3 hours (3 h fraction) after 10km running at maximum speed. The samples were studied by measuring the total protein concentrations, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. The followings were observed；1)Total protein concentrations of the control group (control fraction) were 8.4±7.9 (mean±S.D.) mg/dl. 2)The concentrations of the 5－15 min fraction, 1 h fraction and 3 h fraction were 33.4±43.8mg/dl, 53.3±48.2mg/dl and 10.5±4.8mg/dl, respectively. 3)Statistical significance was；control fraction-5－15 min fraction (p<0.01), control fraction-1 h fraction (p<0.01), 5－15 min fraction-1 h fraction (p<0.05), 1 h fraction-3 h fraction (p<0.01), but no significance was seen between control fraction and 3 h fraction. 4)Urinary excretion protein amount at each fraction after running, and their total amount were also studied. 5－15 min fraction was 10.0±13.1mg, 1 h fraction was 26.7±24.1mg and 3 h fraction 5.2±2.4mg. The total amount of urinary protein excretion of 3 hours after 10 km running was 41.2±29.3mg. 5)Statistical study was done for the urinary protein excretion；5－15 min fraction-1 h fraction (p<0.05), 1 h fraction-3 h fraction (p<0.01), 5－15 min fraction-3 h fraction (no significance). 6)Peaks of total protein concentration were seen at 5－15 min fraction or 1 h fraction. Five protein bands (P1, P2, P3, P4 and P5) were seen by SDS-PAGE in almost all cases. P5 was the main band in the fractions except for the peak fractions. 7) By Western blotting, P1 was α2-macroglobulin, P2 was IgG, P4 was transferrin, P5 was albumin and P3 was the derivative of IgG. IgA was also detected in the position of P2.新潟医学会1997-06jpndepartmental bulletin paperhttp://hdl.handle.net/10191/44972https://niigata-u.repo.nii.ac.jp/records/17341AN0018241500290440新潟医学会雑誌新潟医学会雑誌1116362373https://niigata-u.repo.nii.ac.jp/record/17341/files/111(6)_362-373.pdfapplication/pdf2.0 MB2019-08-08