2024-03-29T14:50:39Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00015963
2022-12-15T03:47:35Z
453:456
471:537:538:1094
習慣流産とヒト組織適合抗原系, 特にクラスII抗原系との関連性 : PCR-RFLP法による解析
習慣流産とヒト組織適合抗原系, 特にクラスII抗原系との関連性 : PCR-RFLP法による解析
Studies of the Relationship Between the HLA-Class II Genotypes and Unexplained Recurrent Abortion-Analysis by Means of PCR-RFLP Method
高桑, 好一
石井, 桂介
安達, 博
今井, 勤
本多, 啓輔
菊池, 朗
荒川, 正人
東野, 昌彦
田中, 憲一
幡谷, 功
田村, 正毅
安田, 雅子
Genotype/HLA-Class II
PCR-RFLP
Recurrent abortion
遺伝子型
HLAクラスⅡ
PCR-RFLP
習慣流産
In Order to elucidate the implication of HLA-class II antigens in the genesis of unexplained recurrent abortion, the frequency of HLA-DRB 1 , -DQB 1 , and -DPB 1 genotypes in patients with unexplained recurrent abortion and the compatibility of the genotypes between patient couples were studied by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Twenty-eight patients with unexplained primary recurrent abortion and their husbands were typed for HLA-DRB 1 , -DQB 1 , and -DPB 1 genotypes. Polymorphic regions at the second exon of HLA-DRB 1 , -DQB 1 , or -DPB 1 were selectively amplified by using the PCR-primers. After amplification, DNAs were digested with restriction endonucleases, and subjected to electrophoresis in a 12% polyacrilamide gel to determine each HLA genotypes. In this study, the frequency of some genotypes was significantly higher in patients group compared with normal population, although no significant compatibility of HLA genotypes could be observed between patient couples compared with normal fertile couples. These data may represent a new class II association with the genesis of unexplained recurrent abortion.
新潟医学会
1999-08
jpn
departmental bulletin paper
http://hdl.handle.net/10191/46791
https://niigata-u.repo.nii.ac.jp/records/15963
AN00182415
00290440
新潟医学会雑誌
新潟医学会雑誌
113
7-8
353
358
https://niigata-u.repo.nii.ac.jp/record/15963/files/113(7-8)_353-358.pdf
application/pdf
858.2 kB
2019-09-30