2024-03-29T09:32:36Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00014773
2022-12-15T03:46:42Z
453:456
471:537:538:1072
C57BL/6マウスES細胞を用いたプルキンエ細胞および時期特異的遺伝子組換え系の開発
C57BL/6マウスES細胞を用いたプルキンエ細胞および時期特異的遺伝子組換え系の開発
Purkinje Cell Specific and Inducible Gene Recombination System Generated from C57BL/6 Mouse ES Cells
北山, 和子
Cerebellar Purkinje cell
spatio-temporally restricted gene targeting
C57BL/6 mouse
ES cell
antiprogesterone
小脳プルキンエ細胞
部位・時期特異的ジーンターゲティング
C57BL/6マウス
胚性幹細胞
アンチプロゲステロン
Spatio-temporally restricted gene targeting is needed for analyzing the functions of various molecules in a variety of biological phenomena. We have generated an inducible cerebellar Purkinje cell-specific gene targeting system. This was achieved by establishing a mutant mouse line (D2CPR) from a C57BL/6 mouse ES cell line, which expressed a fusion protein consisting of the Cre recombinase and the progesterone receptor (CrePR). The Purkinje cell-specific expression of CrePR was attained by inserting CrePR into the glutamate receptor 62 subunit (GluR δ2) gene, which was expressed specifically in the Purkinje cells. Using the transgenic mice carrying the Cre-mediated reporter gene expression, We showed that the antiprogesterone RU486 could induce recombinase activity of the CrePR protein specifically in the mature cerebellar Purkinje cells of the D 2 CPR line. Thus this mutant line will be a useful tool for studying the molecular function of mature Purkinje cells by manipulating gene expression in a temporally restricted manner.
新潟医学会
2001-08
jpn
departmental bulletin paper
http://hdl.handle.net/10191/48356
https://niigata-u.repo.nii.ac.jp/records/14773
AN00182415
00290440
新潟医学会雑誌
新潟医学会雑誌
115
8
397
407
https://niigata-u.repo.nii.ac.jp/record/14773/files/115(8)_397-407.pdf
application/pdf
5.7 MB
2019-08-07