2024-03-29T09:36:30Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00006680
2022-12-15T03:39:59Z
453:456
471:537:568:657
Double minute chromosomes in a 6-MP resistant mouse leukemia L1210 cell line
Double minute chromosomes in a 6-MP resistant mouse leukemia L1210 cell line
Kato, Chihomi
Natsuno, Tetsuya
Saito, Kazuko
6-Mercaptopurine (6-MP) resistant strains of L1210 v/c murine leukemic cells were obtained in vivo 6-MP treatment of a DBA/2 mouse inoculated with sensitive L1210 cells. In one of these 6-MP resistant strains, double minutes (DMs) were found in 12% of the metaphase cells, the number of DMs per cell ranging from 5 to 20. When these 6-MP resistant cells were subcultured in 6-MP free medium for 20 months, only 2% of the cells had DMs ranging from 1 to 5 per cell. The decrease of DMs in number and appearance rate was accompanied by a lowering of the maximum allowable concentration (MAC) of 6-MP necessary to induce resistance in the cells from 1 μg to 0.1 μg/ml. These observations suggest that 6-MP resistance in L1210 cells is the result of an unknown gene amplification.
Niigata University School of Medicine
1987-03
eng
departmental bulletin paper
http://hdl.handle.net/10191/33603
https://niigata-u.repo.nii.ac.jp/records/6680
AA00508361
05677734
Acta medica et biologica
Acta medica et biologica
34
4
111
116
https://niigata-u.repo.nii.ac.jp/record/6680/files/34(4)_111-116.pdf
application/pdf
912.0 kB
2019-08-06