2024-03-29T05:10:28Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00006509
2022-12-15T03:39:49Z
453:456
471:537:568:628
Cholera Toxin B Subunit Shows More Intense Affinity to Schwann Cells than Neurons : In Situ and In Vivo Staining
Cholera Toxin B Subunit Shows More Intense Affinity to Schwann Cells than Neurons : In Situ and In Vivo Staining
Iwanaga, Toshihiko
Ohtsuka, Hiroyuki
Conjugates of horseradish peroxidase (HRP) with cholera toxin (CT) are known to be more effective as a probe for studies of neuronal connectivity than HRP only, because of the affinity of CT for GM1 ganglioside which may be richly expressed on neuronal plasma membranes. The present in situ and in vivo staining using CT-fluorescein isothiocyanate (CT-FITC) and CT-HRP demonstrated their intense binding to Schwann cells rather than neurons. Exogenous GM1 successfully competed for the CT binding sites on Schwann cells. This finding suggests the rich existence of GM1 on the Schwann cell surface.
Niigata University School of Medicine
1994-03
eng
departmental bulletin paper
http://hdl.handle.net/10191/33234
https://niigata-u.repo.nii.ac.jp/records/6509
AA00508361
05677734
Acta medica et biologica
Acta medica et biologica
42
1
19
24
https://niigata-u.repo.nii.ac.jp/record/6509/files/42(1)_19-24.pdf
application/pdf
2.1 MB
2019-08-06