2024-03-28T17:14:58Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00006378
2022-12-15T03:39:39Z
453:456
471:537:568:611
Screening Downstream Genes of a Homeobox Gene by Differential Display Using a Knockout Mouse
Screening Downstream Genes of a Homeobox Gene by Differential Display Using a Knockout Mouse
Nishizawa, Kazumichi
Satokata, Ichiro
Kawano, Yoshiro
Uchiyama, Makoto
homeobox gene
downstream genes
differential display
Msx1
knockout mice
The products of homeobox genes are DNAbinding transcription factors. However, little is known about downstream genes whose activities are regulated directly or indirectly by the homeobox genes. In the current study, we tested a differential display (DD) method using the tissue of a knockout mouse in order to identify the downstream genes of a homeobox gene Msx1 systematically. Our previous in situ hybridization analysis of a Msx1 deficient mouse showed that Msx1 induced by epithelial bone morphogenetic protein 4 (BMP4) and fibroblast growth factors (FGFs) induces Bmp4, the HMG box gene Lef1, and the heparan sulfate proteoglycan syndecan-1 in the tooth mesenchyme. Although it is a very powerful approach for identifying downstream genes of a homeobox gene to test whether the candidate gene's expression is affected in the knockout mouse, this approach is not directly applicable to the identification of unknown genes downstream of Msx1. In the current study, we performed DD using total RNA from E14.5 Msx1 mutant mandibles and were able to obtain four novel downstream genes of Msx1 from 20 cDNA clones verified by Northern blot hybridization and semiquantitative RT-PCR. Despite several problems inherent to this method, we concluded that DD analysis using the tissue of a knockout mouse is a useful systematic approach for the identification of downstream genes of a homeobox gene.
Niigata University School of Medicine
1998-06
eng
departmental bulletin paper
http://hdl.handle.net/10191/33080
https://niigata-u.repo.nii.ac.jp/records/6378
AA00508361
05677734
Acta medica et biologica
Acta medica et biologica
46
2
63
70
https://niigata-u.repo.nii.ac.jp/record/6378/files/46(2)_63-70.pdf
application/pdf
1.3 MB
2019-08-06