2024-03-28T17:13:24Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00006343
2022-12-15T03:39:39Z
453:456
471:537:568:605
Characterization of Low-and High-level Adriamycin-Resistant Human Gastric Cancer Cell Lines: Flow Cytometric Studies and Reversal Effect of Verapamil
Characterization of Low-and High-level Adriamycin-Resistant Human Gastric Cancer Cell Lines: Flow Cytometric Studies and Reversal Effect of Verapamil
UEKI, Kyo
AIZAWA, Kikuo
TANAKA, Shinsuke
NISHIMAKI, Tadashi
SUZUKI, Tsutomu
adriamycin
drug resistance
verapamil
gastric cancer cell line
flow cytometry
We have established two adriamycin (ADM)-resistant sublines with different levels of drug resistance, MKN45R0.05 and MKN45R0.8,from the parental MKN45 human gastric cancer cell line by exposure to stepwise increases in ADM. MKN45R0.05 and MKN45R0.8 cells were 4.8-and 15-fold more resistant to ADM than MKN45 cells, respectively. Flow cytometric (FCM) analysis revealed that MKN45R0.8 cells had significantly lower accumulation of cells in G_2M phase and higher efflux rate of intracellular ADM content than MKN45 and MKN45R0.05 cells. There was no significant increase of P-glycoprotein expression in both resistant sublines by FCM analysis. Verapamil (VPM) showed stronger modifying effects on ADM sensitivity and cell kinetics in MKN45R0.8 than MKN45R0.05 cells. These results indicate that FCM analysis is useful for determining the level of drug resistance, drug accumulation and P-glycoprotein expression in cancer cells, and that the differential resistance-modifying activity of VPM depends on the level of drug resistance.
Niigata University School of Medicine
1999-12
eng
departmental bulletin paper
http://hdl.handle.net/10191/1824
https://niigata-u.repo.nii.ac.jp/records/6343
AA00508361
05677734
Acta medica et biologica
Acta medica et biologica
47
4
161
167
https://niigata-u.repo.nii.ac.jp/record/6343/files/KJ00000007229.pdf
application/pdf
2.5 MB
2019-08-06