2024-03-29T13:34:32Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00006253
2022-12-15T03:39:35Z
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471:537:568:589
T Cell-mediated Induction of Anti-GBM Glomerulonephritis in WKY Rats
T Cell-mediated Induction of Anti-GBM Glomerulonephritis in WKY Rats
MATSUKI, Asako
FUJINAKA, Hidehiko
QU, Zhenylin
ZOU, Jun
EL-SHEMI, Adel Galal Ahmed
KOVALENKO, Pavel
YOSHIDA, Yutaka
YAOITA, Eishin
WILSON, Curtis B.
YAMAMOTO, Tadashi
T cell receptor αβ
anti-GBM glomerulonephritis
CD8^- T cells
WKY rat
Glomerulonephritis induced in Wistar Kyoto (WKY) rats by the intravenous administration of rabbit anti-glomerular basement membrane antibody is characterized by a glomerular accumulation of T lymphocytes and monocytes/macrophages, crescent formation, and massive proteinuria. Although this model was initiated by the antibody injection, the involvement of cell-mediated immunity was also suggested since a crucial role of CD8-positive T lymphocytes was demonstrated in the induction of this model. An implication of humoral and cell-mediated immunity in this model was examined in the present study. The glomerular lesions and urinary protein excretion were deteriorated in rats preimmunized with rabbit IgG or inoculated with rabbit IgG-sensitized rat lymphocytes before the glomerular basement membrane (anti-GBM) antibody administration. In contrast, the anti-GBM glomerulonephritis was ameliorated by the passive transfer of a rat anti-rabbit IgG antibody. These observations suggested that cell-mediated immunity advanced the disease and humoral immunity suppressed it. We then examined the involvement of T cell receptor αβ on T lymphocytes by administrating a monoclonal antibody against the receptor (R73) in rats before or after the anti-GBM antibody injection. Pretreatment with the antibody markedly suppressed the glomerular lesion and proteinuria. However, the suppressive effect was minimal in rats given the antibody after the anti-GBM antibody injection. These results showed that the anti-GBM nephritis in WKY rats was mediated by T cell receptor αβ to initiate cell-mediated immunity.
Niigata University School of Medicine
2004-03
eng
departmental bulletin paper
http://hdl.handle.net/10191/1818
https://niigata-u.repo.nii.ac.jp/records/6253
AA00508361
05677734
Acta medica et biologica
Acta medica et biologica
52
1
11
20
https://niigata-u.repo.nii.ac.jp/record/6253/files/KJ00004267743.pdf
application/pdf
4.4 MB
2019-08-06