2024-03-29T12:40:03Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00006194
2022-12-15T03:39:22Z
453:456
471:537:568:575
Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells
Adenovirus-mediated Gene Transfer of MMP-2 into Cultured Porcine Trabecular Meshwork Cells
Fukuchi, Takeo
Seki, Masaaki
Matsuda, Hidenobu
Ueda, Jun
Abe, Haruki
本文データは学協会の許諾に基づきCiNiiから複製したものである
matrix metalloproteinase-2
adenovirus vector
trabecular meshwork
primary open-angle glaucoma
zymography
This study aimed to use adenoviral gene transfer to express matrix metalloproteinase (MMP)-2 in cultured porcine trabecular meshwork cells and to evaluate the duration of adenovirus-mediated MMP-2 expression and its enzymatic activity. MMP-2 cDNA was synthesized by ligating three segments of MMP-2 cDNA obtained by reverse transcription-polymerase chain reaction (RT-PCR) with mRNA extracted from mouse lungs. MMP-2 cDNA was inserted into replication-deficient adenoviral vectors. Western blotting revealed that MMP-2 was highly expressed by adenoviral gene transfer in cultured porcine trabecular meshwork cells. Zymography confirmed that the expressed MMP-2 possessed enzymatic activity and that MMP-2 activity increased dose-responsively with the viral titer. MMP-2 expression was detected two days after the additional virus preparation and continued for at least three weeks. Adenoviral vectors could efficiently deliver MMP-2 cDNA to cultured trabecular meshwork cells, with MMP-2 gene expression persisting for three weeks after infection. Our data have implications for future gene therapy in glaucoma.
Niigata University School of Medicine
2012-03
eng
departmental bulletin paper
http://hdl.handle.net/10191/20704
https://niigata-u.repo.nii.ac.jp/records/6194
AN1046766X
13458485
Acta medica et biologica
Acta medica et biologica
55
3
81
86
https://niigata-u.repo.nii.ac.jp/record/6194/files/110007138510.pdf
application/pdf
551.3 kB
2019-08-06