2024-03-29T09:52:55Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00002786
2022-12-15T04:33:12Z
453:454
485:486:487
Production of Interspecific Hybrid Plants through Cotyledonary Segment Culture of Embryos Derived from Crosses between Hydrangea macrophylla f. hortensia (Lam.) Rehd. and H. arborescens L.
胚珠培養由来胚の子葉片培養法によるイヨウアジサイとアメリカノリノキとの種間雑種の作出
Kudo, Nobuhiro
Niimi, Yoshiji
園芸学会
Hydrangea macrophylla
H. arborescens
cotyledonary segment culture
interspecific hybrid
A sexual incompatibility mechanism prevents reciprocal crosses between Hydrangea macrophylla f. hortensia (Lam.) Rehd. and H. arborescens L. No viable hybrid plants have been produced by conventional hybridization because the progenies exhibit hybrid lethality at a young seedling stage. For circumventing hybrid lethality, we cultured cotyledonary segments of embryos derived from crosses of H. macrophylla and H. arborescens. The cotyledonary segments formed callus. Fifteen callus lines, which regenerated plantlets, were established from crossings of H. macrophylla 'Blue sky'×H. arborescens 'Annabelle'. However, only a single callus line among them regenerated plantlets which could be transplanted to soil. Hybridity of the regenerated plants was examined by morphological, cytological characteristics, and RAPD markers: morphologically, the regenerated plants appeared intermediate to the parents; the regenerated plants were aneuploids having a chromosome number, 2n=42; whereas those of the parents H. macrophylla 'Blue sky' and H. arborescens 'Annabelle' were 2n=52 and 2n=38, respectively; the RAPD banding patterns of the regenerated plants exhibited the same hybrid bands as the parents. The hybridity of the regenerated plants was probably confirmed by the above methods.
前報でセイヨウアジサイ[Hydrangea macrophylla f. hortensia (Lam.) Rehd.]とアメリカノリノキ(H. arborescens L.)の種間交雑において,交配後の胚珠培養で得られた実生が発芽直後あるいは幼苗期に枯死することを報告した.本研究では,雑種致死を回避し健全な雑種植物を得るため胚珠培養由来胚の子葉片を培養する方法を検討した.セイヨウアジサイ2品種を種子親,アメリカノリノキ1品種を花粉親にした種間交雑で胚珠培養により得た実生の子葉片を植物ホルモン添加培地で培養し,カルスを誘導した.‘ブルースカイ’を種子親にした場合,シュートを分化する15のカルス系統を選抜したが,鉢上げ後ガラス温室で順調に生育したのは1系統のみであった.再分化幼植物の雑種性を検定した結果,形態は両親のほぼ中間を示し,染色体数は,種子親の‘ブルースカイ’は2n=52本,花粉親の‘アナベル’は2n=38本であったのに対し,再分化植物のそれは2n=42本で異数性を示した.さらにRAPDのバンドパターンは両親のバンドを併せもった.以上から再分化植物の雑種性は高いと判断された.
園芸学会
1999-07
eng
journal article
VoR
http://hdl.handle.net/10191/6595
https://niigata-u.repo.nii.ac.jp/records/2786
http://doi.org/10.2503/jjshs.68.803
AN00025257
0013-7626
園芸学会雑誌
68
4
803
809
https://niigata-u.repo.nii.ac.jp/record/2786/files/68(4)_803-809.pdf
application/pdf
1.9 MB
2019-07-29