2024-03-28T17:18:23Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00002528
2022-12-15T04:15:23Z
453:454
485:486:487
Resting spore of plasmodiophora brassicae proliferates only in the callus of clubroot disease-susceptible turnip but increases the PAL activity in the callus of clubroot disease-resistant turnip
Takahashi, Hideyuki
Muraoka, Satoko
Ito, Kimiko
Mitsui, Toshiaki
Hori, Hidetaka
Kiso, Akira
Brassicae campestris L.
callus
clubroot discase resistance and susceptible
PAL
phenylalanine ammonia lyase
Plasmodiophora brassicae
resting spore
turnip
The calluses were indeced from turnips, Brassicae campestris L. which were susceptible and resistant to Plasmodiophora brassicae in Murashige-Skoog’s agar medium supplemented with 1.0 ppm 6-benzylaminopurine and 0.5 ppm α-naphthalene acetic acid. When a 10 μl water suspension containing 104 resting spores of P. brassicae was placed on the surface of the calluses, about 3 × 105 zoosporangium-like spheroids (SLS) were recovered from the susceptible calluses after 24 h of the treatment but no SLS was found in the resistant callus. On 6th day after the treatment, the SLS increased to about 4 × 106 in the susceptible callus. Upon inoculation of resistant callus with 104 resting spores, the phenylalanine ammonia lyase (PAL) activity increased about 4-fold after 20 h, however, such increase was not observed in the susceptible callus during the same period. The consitutive PAL activity of susceptible callus was roughly one 6th of that of resistant callus.
日本植物細胞分子生物学会
2001-09
eng
journal article
VoR
http://hdl.handle.net/10191/17592
https://niigata-u.repo.nii.ac.jp/records/2528
http://doi.org/10.5511/plantbiotechnology.18.267
AA11250821
1342-4580
Plant biotechnology
18
4
267
274
https://niigata-u.repo.nii.ac.jp/record/2528/files/ZL_18(4)_267-274.pdf
application/pdf
1.7 MB
2019-07-29