2024-03-28T13:42:53Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00002524
2022-12-15T03:35:43Z
453:454
468:469:470
Plantlet regeneration from protoplasts of Muscari armeniacum Leichtl. ex Bak.
Nakano, Masaru
Tanaka, Shigefumi
Kagami, Shiho
Saito, Hiroyuki
Adventitious shoot
muscari
plantlet regeneration
protoplast culture
somatic embryo
Protoplasts were isolated from embryogenic calluses of Muscari armeniacum 'Blue Pearl', which had been subcultured for 3 years. Protoplasts started to divide after 5–7 days of culture, and colonies consisting of 50–100 cells were produced after one month. The highest plating efficiency (10.9%) was obtained by using a medium containing 5.4 µM NAA and 4.4 µM BA, 0.5 M glucose and 2 g l−1 gellan gum. Protoplast-derived calluses produced somatic embryos at frequencies of 4.3–89.6% on media containing 0 or 0.54 µM NAA in combination with 0, 4.4, 22 or 44 µM BA, but few embryos converted into plantlets. On the other hand, over 35% of the calluses produced adventitious shoots on media containing 4.4 µM BA or 0.54 µM NAA in combination with 44 µM BA, and some of these shoots developed into plantlets following transfer to a medium without PGRs.
日本植物細胞分子生物学会
2005-09
eng
journal article
VoR
http://hdl.handle.net/10191/29867
https://niigata-u.repo.nii.ac.jp/records/2524
http://doi.org/10.5511/plantbiotechnology.22.249
AA11250821
1342-4580
Plant biotechnology
22
3
249
251
https://niigata-u.repo.nii.ac.jp/record/2524/files/22_3_249-251.pdf
application/pdf
330.1 kB
2019-07-29