2024-03-28T12:45:49Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00002040
2022-12-15T03:34:54Z
453:454
485:486:487
Contractions of Mouse Blastocysts Whose Hatching Abilities were Suppressed by Soybean Trypsin Inhibitor
Niimura, Sueo
Wakasa, Ryoko
Mouse
Blastocyst hatching
Contraction
Soybean trypsin inhibitor
Time-lapse videomicrography
The role of contraction in blastocyst hatching was determined by time-lapse videomicrography in mouse blastocysts whose hatching ability had been suppressed by soybean trypsin inhibitor (STI). The hatching rate of blastocysts developed from morulae in a medium containing STI at a concentration of 1.0 mg/ml (STI-treated blastocysts) was 17.2%, which was significantly lower than the 63.9% for blastocysts developed from morulae in a medium without STI (non-treated blastocysts). Over the span of 32 hrs after blastocoel formation, the number of strong contractions was similar in STI-treated and nontreated blastocysts, but the total number of contractions and the number of weak contractions (less than 20% volume reduction) were significantly smaller in STI-treated blastocysts (4.22 and 2.94 times) than in non-treated blastocysts (5.80 and 4.50 times). These STI-treated blastocysts took a significantly longer time for weak contraction and for re-expansion after weak contraction (10.2 and 87.6 min), compared with non-treated blastocysts (7.8 and 58.2 min). It was also confirmed that the activity of trypsin-like proteinase was similar in STI-treated and non-treated blastocysts, and that a small hole was formed in the zona pellucida at the start of hatching in STI-treated blastocysts, as seen in non-treated blastocysts. Nevertheless, the rate of blastocysts with a slit resulting from enlargement of the small hole in the zona pellucida was 37.5% in STI-treated blastocysts, which was significantly lower than the 86.7% in non-treated blastocysts. These results suggest that weak contraction and re-expansion after weak contraction of blastocysts play an important role in hatching by slitting the region adjacent to the small hole in the zona pellucida.
Japanese Society of Mammalian Ova Research
2001
eng
journal article
VoR
http://hdl.handle.net/10191/7772
https://niigata-u.repo.nii.ac.jp/records/2040
http://doi.org/10.1274/jmor.18.1
AA12018687
1341-7738
Journal of mammalian ova research
18
1
1
7
https://niigata-u.repo.nii.ac.jp/record/2040/files/2009030024.pdf
application/pdf
1.6 MB
2019-07-29