2024-03-29T13:48:28Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00002007
2022-12-15T04:35:36Z
453:454
471:472:473
Prevention of experimental autoimmune myocarditis by hydrodynamics-based naked Plasmid DNA encoding CTLA4-Ig gene delivery
Abe, Satoru
Hanawa, Haruo
Hayashi, Manabu
Yoshida, Tsuyoshi
Komura, Satoru
Watanabe, Ritsuo
Lie, Hui
Chang, He
Kato, Kiminori
Kodama, Makoto
Maruyama, Hiroki
Nakazawa, Mikio
Miyazaki, Junichi
Aizawa, Yoshifusa
Elsevier Inc.
gene therapy
dilated cardiomyopathy
cytokine
pCAGGS
Background: Rat experimental autoimmune myocarditis (EAM) was a T cell-mediated disease, which resembled the giant cell myocarditis seen in humans. Soluble CTLA4 improves some autoimmune diseases by blocking costimulatory signals on T cell. We investigated the effect of hydrodynamics-based naked plasmid DNA encoding CTLA4-immunoglobulin (Ig) gene delivery. Methods and Results: Lewis rats were immunized with cardiac myosin and treated with hydrodynamic-based transfection, namely a rapid tail vein injection of a large volume of pCAGGS encoding CTLA4-Ig chimera solution on Day 0. The vector-derived CTLA4-Ig mRNA expressions were mainly detected in the liver and plasma CTLA4-Ig protein levels were maintained at about 2 μg/ml during the experiment period. On Day 17, the ratio of heart to body weight, the amount of mRNA of atrial natriuretic peptide and the inflammatory areas in CTLA4 group were significantly lower than in the Control group treated with empty plasmid. Maximum rate of intraventricular pressure rise and decline (dp/dt), minimum dp/dt, LVEDP and central venous pressure improved significantly after treatment with CTLA4-Ig. On day 14, expressions of IL-2 in popliteal lymphnodes in the CTLA4-Ig group were significantly lower than in the Control group. Conclusions: Hydrodynamics-based transfection of plasmid encoding CTLA4-Ig chimera dramatically prevented EAM.
2005-09
eng
journal article
http://hdl.handle.net/10191/1161
https://niigata-u.repo.nii.ac.jp/records/2007
16198253
info:doi/10.1016/j.cardfail.2005.04.005
AA11054062
10719164
journal of cardiac failure
journal of cardiac failure
11
7
557
564
https://niigata-u.repo.nii.ac.jp/record/2007/files/22_0001.pdf
application/pdf
608.6 kB
2019-07-29