2024-03-29T01:05:22Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00001646
2022-12-15T04:14:16Z
453:454
471:472:473
The effect of hydrodynamic-based delivery of an interleukin-13-Ig fusion gene for experimental autoimmune myocarditis in rats and its possible mechanism
Elnaggar, Raafat
Hanawa, Haruo
Liu, Hui
Yoshida, Tsuyoshi
Hayashi, Manabu
Watanabe, Ritsuo
Abe, Satoru
Toba, Ken
Yoshida, Kaori
Chang, He
Minagawa, Shiro
Okura, Yuji
Kato, Kiminori
Kodama, Makoto
Maruyama, Hiroki
Miyazaki, Junichi
Aizawa, Yoshifusa
WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
myocarditis
autoimmune
dilated cardiomyopathy
immune system
interleukin-13
gene therapy
Interleukin (IL)-13 is a pleiotropic cytokine secreted by activated Th2-T lymphocytes. Th1 cytokines are assumed to exacerbate while Th2 cytokines to ameliorate rat experimental autoimmune myocarditis (EAM). Here, we examined the effect of IL-13 on EAM using a hydrodynamic-based delivery of an IL-13-Ig and the possible mechanism of its effect. Rats were immunized on day 0 and IL-13-Ig treated rats were injected with pCAGGS-IL-13-Ig and control rats with pCAGGS-Ig on day 1 or 7. On day 17, IL-13-Ig gene therapy was effective in controlling EAM as monitored by the decreased heart weight/body weight ratio, reduced myocarditis and atrial natriuretic peptide mRNA in heart as a heart failure marker. On the basis of IL-13 receptor mRNA expression in separated cells from EAM hearts, we proposed that IL-13-Ig targeting cells were CD11b+ cells and non-cardiomyocytic non-inflammatory (NCNI) cells such as fibroblasts, smooth muscle or endothelial cells. IL-13-Ig inhibited expressing the genes of prostaglandin E synthase, cyclooxygenase-2, inducible nitric oxide synthase, IL-1β and TNFα of cultivated cells from EAM hearts in contrast, while it enhanced IL-1 receptor antagonist. We concluded that IL-13-Ig ameliorates EAM and supposed that its effectiveness may be due to the influence on these immunologic molecules in CD11b+ and NCNI cells.
2005-06
eng
journal article
http://hdl.handle.net/10191/1159
https://niigata-u.repo.nii.ac.jp/records/1646
15902684
info:doi/10.1002/eji.200425776
AA00639610
00142980
European journal of immunology
European journal of immunology
35
6
1995
2005
https://niigata-u.repo.nii.ac.jp/record/1646/files/22_0003.pdf
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2019-07-29